NK Cell, B-Lineage Cell, Dendritic Cell, and Myeloid Progeny From Single Sorted CD34+/Lin−/CD38− Cells Cultured on AFT024 With IL-7, FL, KL, ±IL-3 ± IL-2
| Lineages Derived From Single Cells* . | IL-7, FL, KL† . | IL-7, FL, KL + IL-2† . | IL-7, FL, KL + IL-3† . | IL-7, FL, KL + IL-2 + IL-3† . | ||||
|---|---|---|---|---|---|---|---|---|
| 47/792 (5.9%) Wells Evaluated‡ . | 53/792 (6.7%) Wells Evaluated‡ . | 399/1408 (28%) Wells Evaluated‡ . | 221/880 (25%) Wells Evaluated‡ . | |||||
| Positive (n)2-153/Total (n) . | % Positive . | Positive (n)2-153/Total (n) . | % Positive . | Positive (n)2-153/Total (n) . | % Positive . | Positive (n)2-153/Total (n) . | % Positive . | |
| NK (total) | 8/792 | 1.0 | 21/792 | 2.6 | 172/1,408 | 12.2 | 159/880 | 18.1 |
| DC (total) | 1/792 | 0.1 | 8/792 | 1.0 | 86/880 | 9.8 | 59/352 | 16.7 |
| M (total) | — | — | — | — | 104/352 | 29.5 | 98/352 | 27.8 |
| B (total) | 1/792 | 0.1 | 0/792 | 0 | 29/1,408 | 2.1 | 17/880 | 1.9 |
| NK + DM | 1/792 | 0.1 | 6/792 | 0.7 | 94/1,232 | 7.6 | 107/704 | 15.2 |
| NK/B + DM | 0/792 | 0 | 0/792 | 0 | 19/1,232 | 1.5 | 14/704 | 2.0 |
| Lineages Derived From Single Cells* . | IL-7, FL, KL† . | IL-7, FL, KL + IL-2† . | IL-7, FL, KL + IL-3† . | IL-7, FL, KL + IL-2 + IL-3† . | ||||
|---|---|---|---|---|---|---|---|---|
| 47/792 (5.9%) Wells Evaluated‡ . | 53/792 (6.7%) Wells Evaluated‡ . | 399/1408 (28%) Wells Evaluated‡ . | 221/880 (25%) Wells Evaluated‡ . | |||||
| Positive (n)2-153/Total (n) . | % Positive . | Positive (n)2-153/Total (n) . | % Positive . | Positive (n)2-153/Total (n) . | % Positive . | Positive (n)2-153/Total (n) . | % Positive . | |
| NK (total) | 8/792 | 1.0 | 21/792 | 2.6 | 172/1,408 | 12.2 | 159/880 | 18.1 |
| DC (total) | 1/792 | 0.1 | 8/792 | 1.0 | 86/880 | 9.8 | 59/352 | 16.7 |
| M (total) | — | — | — | — | 104/352 | 29.5 | 98/352 | 27.8 |
| B (total) | 1/792 | 0.1 | 0/792 | 0 | 29/1,408 | 2.1 | 17/880 | 1.9 |
| NK + DM | 1/792 | 0.1 | 6/792 | 0.7 | 94/1,232 | 7.6 | 107/704 | 15.2 |
| NK/B + DM | 0/792 | 0 | 0/792 | 0 | 19/1,232 | 1.5 | 14/704 | 2.0 |
Abbreviations: NK, CD56+ natural killer cell; DC, CD1a+, dendritic cell; M, CD15+ or CD14+ myeloid cell; B, CD19+ B-lineage cell; DM, either CD1a+, CD15+ or CD14+.
Lineage was determined by three-color flow cytometry.
All cytokines added weekly except for IL-3, which was added only once at culture initiation.
Wells determined positive by visual cell growth greater than 100 cells/well were analyzed with a three-color immunophenotype and all other wells were counted as negative.
The number of single cell progeny positive for each lineage or combination of lineages was determined and expressed over the denominator of the total number of single cells plated. All wells were analyzed for NK and B-lineage cells with third fluorescent stain either for DC (CD1a) or M (CD15 or CD14), explaining the lower denominator for these lineages.