AFT024 Supports Differentiation of CD34+/Lin−/DR− Cells Into Both NK Cell and B-Lineage Cell Progeny When Cultured With IL-7, FL, KL, and IL-2
| No. of Starting Cells/Well . | Type of Stromal Feeder . | Cultures Analyzed . | % Wells With NK Cells . | % Wells With B-Lineage Cells . |
|---|---|---|---|---|
| 1,200 | No Stroma | 14 | 0 | 0 |
| 1,200 | M2-10B4 | 14 | 36 | 0 |
| 1,200 | AFT024 | 14 | 100 | 100 |
| 400 | AFT024 | 16 | 100 | 62 |
| No. of Starting Cells/Well . | Type of Stromal Feeder . | Cultures Analyzed . | % Wells With NK Cells . | % Wells With B-Lineage Cells . |
|---|---|---|---|---|
| 1,200 | No Stroma | 14 | 0 | 0 |
| 1,200 | M2-10B4 | 14 | 36 | 0 |
| 1,200 | AFT024 | 14 | 100 | 100 |
| 400 | AFT024 | 16 | 100 | 62 |
Cytokines added at culture initiation with weekly media changes containing IL-2 alone. Cells were obtained from 3 donors. Positive wells were determined by >0.5% staining above controls for CD56 (NK) or CD19/CD10 (B).