Clonality Results for Mobilized PB
| UPN . | Stage . | Day . | % Ph+ . | MNC . | CD34+HLA-DR+ . | CD34+HLA-DR− . | |||
|---|---|---|---|---|---|---|---|---|---|
| BCR/ABL . | CrR . | BCR/ABL . | CrR . | BCR/ABL . | CrR . | ||||
| 1 | ECP | 3 | 30 | + | 0.21 | − | 0.91 | − | 0.98 |
| 2 | ECP | 2 | 15 | + | 0.96 | − | 0.85 | − | 0.88 |
| 4 | ECP | 1 | 904-150 | + | 0.80 | + | 0.74 | + | 0.37 |
| ECP | 3 | 1004-150 | + | 0.08 | + | 0.03 | + | 0.04 | |
| 5 | LCP | 1 | 30 | + | 4.46 | + | 12.77 | + | 9.05 |
| 6 | LCP | 2 | 80 | + | 0.31 | + | 0.19 | + | 0.71 |
| 7 | LCP | 1 | 45 | + | 5.59 | + | 37.71 | + | 16.86 |
| 9 | AP | 5 | 100 | + | 10.31 | + | 15.43 | + | 11.30 |
| UPN . | Stage . | Day . | % Ph+ . | MNC . | CD34+HLA-DR+ . | CD34+HLA-DR− . | |||
|---|---|---|---|---|---|---|---|---|---|
| BCR/ABL . | CrR . | BCR/ABL . | CrR . | BCR/ABL . | CrR . | ||||
| 1 | ECP | 3 | 30 | + | 0.21 | − | 0.91 | − | 0.98 |
| 2 | ECP | 2 | 15 | + | 0.96 | − | 0.85 | − | 0.88 |
| 4 | ECP | 1 | 904-150 | + | 0.80 | + | 0.74 | + | 0.37 |
| ECP | 3 | 1004-150 | + | 0.08 | + | 0.03 | + | 0.04 | |
| 5 | LCP | 1 | 30 | + | 4.46 | + | 12.77 | + | 9.05 |
| 6 | LCP | 2 | 80 | + | 0.31 | + | 0.19 | + | 0.71 |
| 7 | LCP | 1 | 45 | + | 5.59 | + | 37.71 | + | 16.86 |
| 9 | AP | 5 | 100 | + | 10.31 | + | 15.43 | + | 11.30 |
Data are shown for those days of the PBPC harvest where interpretable BCR/ABL and HUMARA results could be obtained in all individual cell fractions. Determination of the percent of Ph+ metaphases was performed on mononuclear cells by metaphase fluorescence in situ hybridization. BCR/ABL was scored as positive (+) if at least one of both RT-PCR reactions was positive, and scored as negative (−) if no BCR/ABL signal could be detected after Southern blotting despite positivity for β-actin. Calculation of mean CrR values for the HUMARA locus was performed as described in Materials and Methods.
Metaphases of UPN 4 showed a complex translocation involving chromosomes 9, 17, and 22.