Immunohistochemical Staining of Peritoneal and Bronchoalveolar Lavage Specimens From WT and βc Null Mice
| . | Peritoneal Macrophages . | Alveolar Macrophages . | |||
|---|---|---|---|---|---|
| WT . | βc Null . | WT . | βc Null . | ||
| Typical . | Large . | ||||
| F4/80 | + | + | − | + | + |
| Macrosialin/FA-11 | +/− | + | + | + | + |
| Sialoadhesin/IC2 | − | − | + | +/− | +/− |
| mMSR/2F8 | + | + | + | + | + |
| CR3/β2 integrin/5C6 | + | + | − | + | + |
| CD44/8D2 | + | +/− | ND | + | + |
| MHC class II/TIB 120 | − | − | − | − | + |
| . | Peritoneal Macrophages . | Alveolar Macrophages . | |||
|---|---|---|---|---|---|
| WT . | βc Null . | WT . | βc Null . | ||
| Typical . | Large . | ||||
| F4/80 | + | + | − | + | + |
| Macrosialin/FA-11 | +/− | + | + | + | + |
| Sialoadhesin/IC2 | − | − | + | +/− | +/− |
| mMSR/2F8 | + | + | + | + | + |
| CR3/β2 integrin/5C6 | + | + | − | + | + |
| CD44/8D2 | + | +/− | ND | + | + |
| MHC class II/TIB 120 | − | − | − | − | + |
Antibodies against a range of macrophage markers and adhesion molecules were tested. In each case samples incubated with the isotype control antibody were unstained.
Abbreviations: −, no staining detected; ND, not done.