Cumulative Growth Kinetics Over 7 Days and Phenotype of Daughter Cells After the First Division of Single CD34+Thy-1+lin− Cells
| Culture Stimulation . | Cumulative % Wells With 1 Live Cell (over 7 d) . | Cumulative % Wells in Which Cell Died (over 7 d) . | Cumulative % Wells in Which Cell Doubled (over 7 d) . | % Doublets Conserved Phenotype Both Cells (34+lin−) . | % Doublets With 1 Daughter Cell Conserved Phenotype (34+lin−) . | % Doublets Differentiated Phenotype Both Cells (34+lin+) . |
|---|---|---|---|---|---|---|
| FLT-3L | 22.1 | 53.3 | 24.6 | 18.8 ± 11.96 | 27.9 ± 7.55 | 53.3 ± 9.72 |
| TPO | 29.4 | 35.3 | 35.3 | 28.5 ± 7.98 | 34.0 ± 5.41 | 37.5 ± 12.5 |
| IL-1 | 20.6 | 66.3 | 13.0 | 21.5 ± 21.45 | 57.1 ± 42.85 | 21.5 ± 21.45 |
| IL-6 | 13.1 | 68.7 | 18.2 | 38.3 ± 7.26 | 46.7 ± 3.33 | 15.0 ± 7.63 |
| IL-11 | 13.6 | 68.2 | 18.2 | 0 ± 0 | 37.5 ± 37.50 | 62.5 ± 37.50 |
| HGF | 15.4 | 64.1 | 20.5 | 0 ± 0 | 37.5 ± 37.50 | 62.5 ± 37.50 |
| SCF (50 ng) | 26.0 | 51.0 | 23.0 | 25.0 ± 0 | 31.3 ± 6.25 | 43.8 ± 6.25 |
| SCF (100 ng) | 20.0 | 59.0 | 21.0 | 17.8 ± 9.67 | 38.9 ± 12.51 | 43.3 ± 12.01 |
| FLT-3L + TPO | 10.0 | 27.0 | 63.0 | 56.8 ± 6.80 | 15.2 ± 6.20 | 28.0 ± 0.65 |
| SCF + TPO | 1.2 | 30.9 | 67.9 | 38.5 ± 13.50 | 23.1 ± 16.87 | 38.4 ± 30.35 |
| FLT-3L + HGF | 21.9 | 50.0 | 28.1 | 20.0 ± 20.00 | 35.5 ± 24.55 | 44.4 ± 44.4 |
| FLT-3L, SCF, IL-3, IL-6, G-CSF, βNGF | 5.18 | 55.4 | 39.4 | 39.0 ± 10.72 | 27.9 ± 4.34 | 33.1 ± 13.8 |
| SCF, IL-3, IL-6, G-CSF, GM-CSF | 6.8 | 53.7 | 39.5 | 35.8 ± 4.93 | 33.7 ± 14.10 | 30.5 ± 10.43 |
| HGF, IL-1, IL-6, G-CSF | 14.3 | 70.3 | 15.4 | 6.7 ± 6.66 | 74.4 ± 17.87 | 18.9 ± 11.62 |
| No factors | 21.7 | 76.1 | 2.2 | 0 ± 0 | 100 ± 0 | 0 ± 0 |
| Culture Stimulation . | Cumulative % Wells With 1 Live Cell (over 7 d) . | Cumulative % Wells in Which Cell Died (over 7 d) . | Cumulative % Wells in Which Cell Doubled (over 7 d) . | % Doublets Conserved Phenotype Both Cells (34+lin−) . | % Doublets With 1 Daughter Cell Conserved Phenotype (34+lin−) . | % Doublets Differentiated Phenotype Both Cells (34+lin+) . |
|---|---|---|---|---|---|---|
| FLT-3L | 22.1 | 53.3 | 24.6 | 18.8 ± 11.96 | 27.9 ± 7.55 | 53.3 ± 9.72 |
| TPO | 29.4 | 35.3 | 35.3 | 28.5 ± 7.98 | 34.0 ± 5.41 | 37.5 ± 12.5 |
| IL-1 | 20.6 | 66.3 | 13.0 | 21.5 ± 21.45 | 57.1 ± 42.85 | 21.5 ± 21.45 |
| IL-6 | 13.1 | 68.7 | 18.2 | 38.3 ± 7.26 | 46.7 ± 3.33 | 15.0 ± 7.63 |
| IL-11 | 13.6 | 68.2 | 18.2 | 0 ± 0 | 37.5 ± 37.50 | 62.5 ± 37.50 |
| HGF | 15.4 | 64.1 | 20.5 | 0 ± 0 | 37.5 ± 37.50 | 62.5 ± 37.50 |
| SCF (50 ng) | 26.0 | 51.0 | 23.0 | 25.0 ± 0 | 31.3 ± 6.25 | 43.8 ± 6.25 |
| SCF (100 ng) | 20.0 | 59.0 | 21.0 | 17.8 ± 9.67 | 38.9 ± 12.51 | 43.3 ± 12.01 |
| FLT-3L + TPO | 10.0 | 27.0 | 63.0 | 56.8 ± 6.80 | 15.2 ± 6.20 | 28.0 ± 0.65 |
| SCF + TPO | 1.2 | 30.9 | 67.9 | 38.5 ± 13.50 | 23.1 ± 16.87 | 38.4 ± 30.35 |
| FLT-3L + HGF | 21.9 | 50.0 | 28.1 | 20.0 ± 20.00 | 35.5 ± 24.55 | 44.4 ± 44.4 |
| FLT-3L, SCF, IL-3, IL-6, G-CSF, βNGF | 5.18 | 55.4 | 39.4 | 39.0 ± 10.72 | 27.9 ± 4.34 | 33.1 ± 13.8 |
| SCF, IL-3, IL-6, G-CSF, GM-CSF | 6.8 | 53.7 | 39.5 | 35.8 ± 4.93 | 33.7 ± 14.10 | 30.5 ± 10.43 |
| HGF, IL-1, IL-6, G-CSF | 14.3 | 70.3 | 15.4 | 6.7 ± 6.66 | 74.4 ± 17.87 | 18.9 ± 11.62 |
| No factors | 21.7 | 76.1 | 2.2 | 0 ± 0 | 100 ± 0 | 0 ± 0 |
Phenotype values are mean ± SEM (represents differences in individual cord responsiveness).