Growth Kinetics of Single CD34+lin− Cells in Cultures Supplemented With FLT-3L + HGF
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 90.3 ± 1.85 | 6.5 ± 1.15 | 3.2 ± 0.60 |
| 3 | 63.6 ± 5.65 | 16.9 ± 1.50 | 9.8 ± 6.00 |
| 4 | 52.5 ± 5.10 | 13 ± 1.30 | 9.7 ± 1.80 |
| 5 | 37.0 ± 5.35 | 1.9 ± 1.90 | 13.7 ± 2.15 |
| 6 | 28.4 ± 10.00 | 1.3 ± 1.30 | 7.2 ± 3.35 |
| 7 | 10.5 | 0 | 7.9 |
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 90.3 ± 1.85 | 6.5 ± 1.15 | 3.2 ± 0.60 |
| 3 | 63.6 ± 5.65 | 16.9 ± 1.50 | 9.8 ± 6.00 |
| 4 | 52.5 ± 5.10 | 13 ± 1.30 | 9.7 ± 1.80 |
| 5 | 37.0 ± 5.35 | 1.9 ± 1.90 | 13.7 ± 2.15 |
| 6 | 28.4 ± 10.00 | 1.3 ± 1.30 | 7.2 ± 3.35 |
| 7 | 10.5 | 0 | 7.9 |
Single CD34+lin− cells were plated at 1 cell/well in serum-free medium and monitored over 7 days as described in Materials and Methods. Data represent the means ± SEM of 2 experiments with a total of 64 wells containing a single cell.