Growth Kinetics of Single CD34+lin− Cells in Cultures Supplemented With SCF, IL-3, IL-6, G-CSF, GM-CSF
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 89.3 ± 2.87 | 10.2 ± 2.87 | 0 ± 0 |
| 3 | 61.7 ± 8.71 | 23.7 ± 6.40 | 4.5 ± 2.31 |
| 4 | 39.4 ± 7.28 | 4.7 ± 2.01 | 17.5 ± 2.68 |
| 5 | 21.6 ± 6.35 | 0.97 ± 0.96 | 16.9 ± 0.96 |
| 6 | 9.5 ± 5.51 | 1.03 ± 1.03 | 11.1 ± 4.58 |
| 7 | 6.4 ± 3.12 | 0 ± 0 | 3.1 ± 2.40 |
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 89.3 ± 2.87 | 10.2 ± 2.87 | 0 ± 0 |
| 3 | 61.7 ± 8.71 | 23.7 ± 6.40 | 4.5 ± 2.31 |
| 4 | 39.4 ± 7.28 | 4.7 ± 2.01 | 17.5 ± 2.68 |
| 5 | 21.6 ± 6.35 | 0.97 ± 0.96 | 16.9 ± 0.96 |
| 6 | 9.5 ± 5.51 | 1.03 ± 1.03 | 11.1 ± 4.58 |
| 7 | 6.4 ± 3.12 | 0 ± 0 | 3.1 ± 2.40 |
Single CD34+lin− cells were plated at 1 cell/well in serum-free medium and monitored over 7 days as described in Materials and Methods. Data represent the means ± SEM of 3 experiments with a total of 177 wells containing a single cell.