Growth Kinetics of Single CD34+lin− Cells in Cultures Supplemented With HGF, IL-1, IL11, G-CSF
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 93.6 ± 3.05 | 6.4 ± 3.05 | 0 ± 0 |
| 3 | 71.4 ± 15.18 | 5.1 ± 0.84 | 17.1 ± 12.92 |
| 4 | 47.7 ± 17.35 | 2.2 ± 1.12 | 21.5 ± 5.93 |
| 5 | 33.1 ± 18.47 | 0.5 ± 0.53 | 14.0 ± 10.60 |
| 6 | 22.9 ± 17.63 | 0.5 ± 0.53 | 9.7 ± 4.53 |
| 7 | 13.5 ± 12.61 | 0.5 ± 0.53 | 8.9 ± 4.45 |
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 93.6 ± 3.05 | 6.4 ± 3.05 | 0 ± 0 |
| 3 | 71.4 ± 15.18 | 5.1 ± 0.84 | 17.1 ± 12.92 |
| 4 | 47.7 ± 17.35 | 2.2 ± 1.12 | 21.5 ± 5.93 |
| 5 | 33.1 ± 18.47 | 0.5 ± 0.53 | 14.0 ± 10.60 |
| 6 | 22.9 ± 17.63 | 0.5 ± 0.53 | 9.7 ± 4.53 |
| 7 | 13.5 ± 12.61 | 0.5 ± 0.53 | 8.9 ± 4.45 |
Single CD34+lin− cells were plated at 1 cell/well in serum-free medium and monitored over 7 days as described in Materials and Methods. Data represent the means ± SEM of 3 experiments with a total of 175 wells containing a single cell.