Growth Kinetics of Single CD34+lin− Cells in Cultures Supplemented With SCF + TPO
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 88.7 ± 1.95 | 11.3 ± 1.95 | 0 ± 0 |
| 3 | 34.7 ± 2.10 | 47.7 ± 8.15 | 6.4 ± 4.10 |
| 4 | 16.2 ± 2.20 | 3.5 ± 3.50 | 15.0 ± 3.40 |
| 5 | 5.6 ± 3.35 | 2.5 ± 0.15 | 7.8 ± 5.45 |
| 6 | 2.4 ± 2.35 | 2.4 ± 2.35 | 0 ± 0 |
| 7 | 1.2 ± 1.15 | 0 ± 0 | 1.2 ± 1.15 |
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 88.7 ± 1.95 | 11.3 ± 1.95 | 0 ± 0 |
| 3 | 34.7 ± 2.10 | 47.7 ± 8.15 | 6.4 ± 4.10 |
| 4 | 16.2 ± 2.20 | 3.5 ± 3.50 | 15.0 ± 3.40 |
| 5 | 5.6 ± 3.35 | 2.5 ± 0.15 | 7.8 ± 5.45 |
| 6 | 2.4 ± 2.35 | 2.4 ± 2.35 | 0 ± 0 |
| 7 | 1.2 ± 1.15 | 0 ± 0 | 1.2 ± 1.15 |
Single CD34+lin− cells were plated at 1 cell/well in serum-free medium and monitored over 7 days as described in Materials and Methods. Data represent the means ± SEM of 2 experiments with a total of 77 wells containing a single cell.