Growth Kinetics of Single CD34+lin− Cells in Cultures Supplemented With FLT-3L + TPO
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 76.0 ± 5.85 | 24.0 ± 5.80 | 0 ± 0 |
| 3 | 54.9 ± 0.35 | 18.1 ± 3.15 | 3.1 ± 3.05 |
| 4 | 36.1 ± 0.30 | 15.8 ± 3.65 | 3.1 ± 3.05 |
| 5 | 20.2 ± 8.10 | 0.8 ± 0.75 | 15.1 ± 9.15 |
| 6 | 15.8 ± 6.65 | 2.3 ± 0.75 | 2.3 ± 2.25 |
| 7 | 9.8 ± 0.65 | 0 ± 0 | 5.6 ± 5.95 |
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 76.0 ± 5.85 | 24.0 ± 5.80 | 0 ± 0 |
| 3 | 54.9 ± 0.35 | 18.1 ± 3.15 | 3.1 ± 3.05 |
| 4 | 36.1 ± 0.30 | 15.8 ± 3.65 | 3.1 ± 3.05 |
| 5 | 20.2 ± 8.10 | 0.8 ± 0.75 | 15.1 ± 9.15 |
| 6 | 15.8 ± 6.65 | 2.3 ± 0.75 | 2.3 ± 2.25 |
| 7 | 9.8 ± 0.65 | 0 ± 0 | 5.6 ± 5.95 |
Single CD34+lin− cells were plated at 1 cell/well in serum-free medium and monitored over 7 days as described in Materials and Methods. Data represent the means ± SEM of 2 experiments with a total of 100 wells containing a single cell.