Growth Kinetics of Single CD34+lin− Cells in Cultures Supplemented With IL-1
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 89.3 ± 4.23 | 3.3 ± 1.69 | 7.4 ± 4.30 |
| 3 | 70.3 ± 6.51 | 8.6 ± 8.63 | 10.6 ± 3.84 |
| 4 | 46.9 ± 13.21 | 1.2 ± 1.23 | 21.9 ± 7.44 |
| 5 | 39.9 ± 9.30 | 0 ± 0 | 7.0 ± 5.00 |
| 6 | 37.2 ± 10.53 | 1.2 ± 1.23 | 1.4 ± 1.43 |
| 7 | 23.6 ± 10.91 | 0 ± 0 | 13.6 ± 8.63 |
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 89.3 ± 4.23 | 3.3 ± 1.69 | 7.4 ± 4.30 |
| 3 | 70.3 ± 6.51 | 8.6 ± 8.63 | 10.6 ± 3.84 |
| 4 | 46.9 ± 13.21 | 1.2 ± 1.23 | 21.9 ± 7.44 |
| 5 | 39.9 ± 9.30 | 0 ± 0 | 7.0 ± 5.00 |
| 6 | 37.2 ± 10.53 | 1.2 ± 1.23 | 1.4 ± 1.43 |
| 7 | 23.6 ± 10.91 | 0 ± 0 | 13.6 ± 8.63 |
Single CD34+lin− cells were plated at 1 cell/well in serum-free medium and monitored over 7 days as described in Materials and Methods. Data represent the means ± SEM of 3 experiments with a total of 92 wells containing a single cell.