Growth Kinetics of Single CD34+lin− Cells in Cultures Supplemented With SCF (100 ng/mL)
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 95.6 ± 2.28 | 2.2 ± 1.12 | 2.2 ± 1.12 |
| 3 | 73.7 ± 6.13 | 8.7 ± 4.56 | 13.2 ± 2.57 |
| 4 | 48.7 ± 7.16 | 7.6 ± 2.03 | 17.3 ± 7.59 |
| 5 | 39.1 ± 10.22 | 0.9 ± 0.9 | 8.7 ± 3.67 |
| 6 | 27.9 ± 5.47 | 0.9 ± 0.9 | 10.3 ± 4.49 |
| 7 | 20.7 ± 3.215-150 | 0.9 ± 0.9 | 6.3 ± 6.3 |
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 95.6 ± 2.28 | 2.2 ± 1.12 | 2.2 ± 1.12 |
| 3 | 73.7 ± 6.13 | 8.7 ± 4.56 | 13.2 ± 2.57 |
| 4 | 48.7 ± 7.16 | 7.6 ± 2.03 | 17.3 ± 7.59 |
| 5 | 39.1 ± 10.22 | 0.9 ± 0.9 | 8.7 ± 3.67 |
| 6 | 27.9 ± 5.47 | 0.9 ± 0.9 | 10.3 ± 4.49 |
| 7 | 20.7 ± 3.215-150 | 0.9 ± 0.9 | 6.3 ± 6.3 |
Single CD34+lin− cells were plated at 1 cell/well in serum-free medium and monitored over 7 days as described in Materials and Methods. Data represent the means ± SEM of 3 experiments with a total of 100 wells containing a single cell.
P ≤ .05 significantly different from all cytokines for the number of cells tested except SCF 50 ng/mL, HGF, FTL-3L.