Growth Kinetics of Single CD34+lin− Cells in Cultures With No-Added Factors
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 86.1 ± 9.90 | 0 ± 0 | 13.9 ± 9.90 |
| 3 | 65.2 ± 12.80 | 0 ± 0 | 20.9 ± 2.90 |
| 4 | 43.9 ± 8.15 | 0.5 ± 0.50 | 20.4 ± 3.65 |
| 5 | 30.2 ± 6.35 | 0.5 ± 0.50 | 12.0 ± 0.05 |
| 6 | 22.2 ± 7.85 | 0 ± 0 | 8.8 ± 0.75 |
| 7 | 11.9 | 0 | 2.4 |
| Day . | % Wells With 1 Live Cell . | % Wells in Which Cell Doubled . | % Wells in Which Cell Died . |
|---|---|---|---|
| 1 | 100 | — | — |
| 2 | 86.1 ± 9.90 | 0 ± 0 | 13.9 ± 9.90 |
| 3 | 65.2 ± 12.80 | 0 ± 0 | 20.9 ± 2.90 |
| 4 | 43.9 ± 8.15 | 0.5 ± 0.50 | 20.4 ± 3.65 |
| 5 | 30.2 ± 6.35 | 0.5 ± 0.50 | 12.0 ± 0.05 |
| 6 | 22.2 ± 7.85 | 0 ± 0 | 8.8 ± 0.75 |
| 7 | 11.9 | 0 | 2.4 |
Single CD34+lin− cells were plated at 1 cell/well in serum-free medium and monitored over 7 days as described in Materials and Methods. Data represent the means ± SEM of 2 experiments with a total of 92 wells containing a single cell.