Table 2. AT Protein Expression by... | American Society of Hematology

Table 2.

AT Protein Expression by Reticulocyte Lysate and COS 7 Cells

	Hydrophobicity^*	COS 7		Cell-Free
	Hydrophobicity^*	Lysate	Supernatant	Translation	Processed^†
Wild type	−1.8	+	+	+	+
L(−10)V	−1.5	+	+	+	+
L(−10)M	−1.3	+	+	+	+
L(−10)T	−0.4	+	−	+	−
L(−10)P	0.0	+	−	+	−
L(−10)G	0.0	+	−	+	−
L(−10)R	3.0	+	−	+	−
L(−10)Del	−1.8^‡	+	+	+	+/−

	Hydrophobicity^*	COS 7		Cell-Free
	Hydrophobicity^*	Lysate	Supernatant	Translation	Processed^†
Wild type	−1.8	+	+	+	+
L(−10)V	−1.5	+	+	+	+
L(−10)M	−1.3	+	+	+	+
L(−10)T	−0.4	+	−	+	−
L(−10)P	0.0	+	−	+	−
L(−10)G	0.0	+	−	+	−
L(−10)R	3.0	+	−	+	−
L(−10)Del	−1.8^‡	+	+	+	+/−

AT protein assessed as being present (+) or absent (−).

Hydrophobicity of the residue at position −10, according to Hopp and Woods.29

Processing by microsomes (see Materials and Methods).

Deletion results in I becoming the −10 residue.