Table 1. Primers for Site-Directed... | American Society of Hematology

Table 1.

Primers for Site-Directed Mutagenesis

Primer	Sequence^*
L(−10)P forward	CGATCGGCTTCTGGGACTGCGTGA
L(−10)P reverse	GCAGCAAGGACAAAAGATAAACC
(−10)Del forward	ATTGGCTTCTGGGACTGCGTG
L(−10)M forward	ATCATTGGCTTCTGGGACTGCGTG
L(−10)V forward	GTCATTGGCTTCTGGGACTGCGTG
L(−10)T forward	ACCATTGGCTTCTGGGACTGCGTG
L(−10)G forward	GGCATTGGCTTCTGGGACTGCGTG
L(−10)R forward	CGCATTGGCTTCTGGGACTGCGTG
L(−10) common reverse	CAGCAAGGACAAAAGATAAAC

Primer	Sequence^*
L(−10)P forward	CGATCGGCTTCTGGGACTGCGTGA
L(−10)P reverse	GCAGCAAGGACAAAAGATAAACC
(−10)Del forward	ATTGGCTTCTGGGACTGCGTG
L(−10)M forward	ATCATTGGCTTCTGGGACTGCGTG
L(−10)V forward	GTCATTGGCTTCTGGGACTGCGTG
L(−10)T forward	ACCATTGGCTTCTGGGACTGCGTG
L(−10)G forward	GGCATTGGCTTCTGGGACTGCGTG
L(−10)R forward	CGCATTGGCTTCTGGGACTGCGTG
L(−10) common reverse	CAGCAAGGACAAAAGATAAAC

With mutations underlined.