Percentages of Cells Displaying Morphologic Characteristics of Apoptosis and PS Exposure in Untreated and Anti-Fas MoAb-Treated Neutrophils
| Treatment . | % Apoptotic Morphology . | % Annexin V-FITC Fluorescence . |
|---|---|---|
| Medium alone | ||
| 3 h | 2.1 ± 1.1 | 5.3 ± 2.0 |
| 6 h | 8.4 ± 2.2 | 12.3 ± 4.1 |
| 24 h | 48.5 ± 4.3 | 55.0 ± 6.2 |
| Anti-Fas MoAb (250 ng/mL) | ||
| 3 h | 4.4 ± 1.8 | 6.8 ± 3.2 |
| 6 h | 17.2 ± 4.5 | 22.7 ± 4.8 |
| 24 h | 68.2 ± 8.0 | 72.9 ± 9.4 |
| Treatment . | % Apoptotic Morphology . | % Annexin V-FITC Fluorescence . |
|---|---|---|
| Medium alone | ||
| 3 h | 2.1 ± 1.1 | 5.3 ± 2.0 |
| 6 h | 8.4 ± 2.2 | 12.3 ± 4.1 |
| 24 h | 48.5 ± 4.3 | 55.0 ± 6.2 |
| Anti-Fas MoAb (250 ng/mL) | ||
| 3 h | 4.4 ± 1.8 | 6.8 ± 3.2 |
| 6 h | 17.2 ± 4.5 | 22.7 ± 4.8 |
| 24 h | 68.2 ± 8.0 | 72.9 ± 9.4 |
Freshly isolated neutrophils were evaluated for morphology and degree of PS exposure as outlined in the legend to Fig 1. Data are shown as mean ± SD (n = 4).