Table 3. Repopulation of EGFP-Transduced... | American Society of Hematology

Table 3.

Repopulation of EGFP-Transduced UCB Subsets in NOD/SCID Mice and CAFC Assay

Vector/Packaging Cell Line	UCB Subset	Transduction Efficiency % EGFP	EGFP⁺/Chimeric Mice^3-150	Chimerism in NOD/SCID % CD45	EGFP⁺ on CD45⁺ Cells %	CAFC wk 6 % EGFP
MFG-EGFP/Am12	CD34⁺	31^3-151 (29-51)	6/10	12 (2-65)	2 (0-18)	6 (0-11)
SF-EGFP/PG13	CD34⁺	66^3-151	4/4	8 (3-12)	23 (2-41)	27 (26-27)
P value		—	—	>.05	.032	.12
MFG-EGFP/Am12	CD34⁺CD38⁻	5^3-151	0/4	5 (1-24)	0	ND
SF-EGFP/PG13	CD34⁺CD38⁻	21^3-151	1/3	6 (4-9)	3	25^3-151
P value	—	—	>.05	>.05	—

Vector/Packaging Cell Line	UCB Subset	Transduction Efficiency % EGFP	EGFP⁺/Chimeric Mice^3-150	Chimerism in NOD/SCID % CD45	EGFP⁺ on CD45⁺ Cells %	CAFC wk 6 % EGFP
MFG-EGFP/Am12	CD34⁺	31^3-151 (29-51)	6/10	12 (2-65)	2 (0-18)	6 (0-11)
SF-EGFP/PG13	CD34⁺	66^3-151	4/4	8 (3-12)	23 (2-41)	27 (26-27)
P value		—	—	>.05	.032	.12
MFG-EGFP/Am12	CD34⁺CD38⁻	5^3-151	0/4	5 (1-24)	0	ND
SF-EGFP/PG13	CD34⁺CD38⁻	21^3-151	1/3	6 (4-9)	3	25^3-151
P value	—	—	>.05	>.05	—

Results are depicted as median (range) of 2 or 3 experiments. For statistical analysis the Mann-Whitney U-test has been used.

Abbreviation: ND, not detectable.

All transplanted mice engrafted with >1% CD45⁺ cells.

Insufficient data to perform statistical analysis.