Chloride Transport Data
| . | K562/B3 Clone 1 . | RBC . |
|---|---|---|
| Mean cell volume (μm3) | 2,360 | 78 |
| Mean cell surface area (μm2) | 1,840 | 142 |
| Intracellular Cl− concentration (mmol/L) | 84 | 115 |
| Rate constant for band 3-mediated chloride efflux °kCl− at 5°C (s−1) | −0.144 × 10−2 | −0.103 |
| Relative chloride flux °MCl− at 5°C (mmol · μm−2 · s−1 × 10−16) | 1.55 | 64.8 |
| Rate constant for band 3–mediated chloride efflux °kCl− at 20°C (s−1) | −2.066 × 10−2 | −1.430 |
| Relative chloride flux °MCl− at 20°C (mmol · μm−2 · s−1 × 10−16) | 22.3 | 901 |
| . | K562/B3 Clone 1 . | RBC . |
|---|---|---|
| Mean cell volume (μm3) | 2,360 | 78 |
| Mean cell surface area (μm2) | 1,840 | 142 |
| Intracellular Cl− concentration (mmol/L) | 84 | 115 |
| Rate constant for band 3-mediated chloride efflux °kCl− at 5°C (s−1) | −0.144 × 10−2 | −0.103 |
| Relative chloride flux °MCl− at 5°C (mmol · μm−2 · s−1 × 10−16) | 1.55 | 64.8 |
| Rate constant for band 3–mediated chloride efflux °kCl− at 20°C (s−1) | −2.066 × 10−2 | −1.430 |
| Relative chloride flux °MCl− at 20°C (mmol · μm−2 · s−1 × 10−16) | 22.3 | 901 |
Data for RBCs are according to Brahm.54 The extracellular chloride concentration is approximately 145 to 150 mmol/L for all efflux measurements. The rate constant for band 3–mediated efflux in K562/B3 clone 1 was calculated as the difference between the rate constant for K562/B3 clone 1 without DNDS and the rate constant for K562/pBp without DNDS.