Table 1. Protocol for BMT Experiments... | American Society of Hematology

Table 1.

Protocol for BMT Experiments

Experiment	Donor	Recipient	No. of Cells Injected^*	RBC Suppl. (+ or −)^†	Irradiation^‡	Assessment^1-153
BMTI
+/+ → +/+^a	+/+	+/+^a	3 × 10⁶	+	10 Gy	GPI-1 conversion
sph/sph → +/+^a	sph/sph	+/+^a	3 × 10⁶	+	10 Gy	GPI-1 conversion
+/+ → W/W^v	+/+	W/W^v	3 × 10⁶	−	−	RBC counts
sph/sph → W/W^v	sph/sph	W/W^v	3 × 10⁶	−	−	RBC counts
BMTII
+/+ → +/+^a	+/+	+/+^a	2.5 × 10⁶	+	11.5 Gy	GPI-1 conversion
sph/sph → +/+^a	sph/sph	+/+^a	2.5 × 10⁶	+	11.5 Gy	GPI-1 conversion
+/+ → W/W^v	+/+	W/W^v	2.5 × 10⁶	−	−	RBC counts
sph/sph → W/W^v	sph/sph	W/W^v	2.5 × 10⁶	−	−	RBC counts

Experiment	Donor	Recipient	No. of Cells Injected^*	RBC Suppl. (+ or −)^†	Irradiation^‡	Assessment^1-153
BMTI
+/+ → +/+^a	+/+	+/+^a	3 × 10⁶	+	10 Gy	GPI-1 conversion
sph/sph → +/+^a	sph/sph	+/+^a	3 × 10⁶	+	10 Gy	GPI-1 conversion
+/+ → W/W^v	+/+	W/W^v	3 × 10⁶	−	−	RBC counts
sph/sph → W/W^v	sph/sph	W/W^v	3 × 10⁶	−	−	RBC counts
BMTII
+/+ → +/+^a	+/+	+/+^a	2.5 × 10⁶	+	11.5 Gy	GPI-1 conversion
sph/sph → +/+^a	sph/sph	+/+^a	2.5 × 10⁶	+	11.5 Gy	GPI-1 conversion
+/+ → W/W^v	+/+	W/W^v	2.5 × 10⁶	−	−	RBC counts
sph/sph → W/W^v	sph/sph	W/W^v	2.5 × 10⁶	−	−	RBC counts

Number of bone marrow cells injected in a 0.1 mL volume.

Supplementation of bone marrow cells with peripheral RBCs from a B6 donor.

Total irradiation dose per mouse; “−” indicates no irradiation.

Method used to assess implantation of donor marrow.