Bone marrow samples were recovered from bnx mice after engraftment periods of 6 to 12 months. Human-specific colony-forming assays with and without addition of the selective agent G418 were plated from the bone marrow of each mouse, and the results are shown as: no. of G418-resistant/total human CFU (% G418 − resistant CFU). PCR for the presence of the neogene in the LN proviral vector was performed on each marrow sample. Results of the PCR analyses are indicated as positive (+) or noneo gene detected (neg). Genomic DNA samples extracted from the marrow of each mouse that carried neo marked cells were further subjected to clonal analysis by inverse PCR. The number of proviral integrants detected in each sample (no. of PCR product bands/2 LTR bands per proviral integrant = no. of integrants) is listed. Individual human T-lymphoid cells (CD45⁺/CD3⁺) and myeloid progenitors (CD45⁺/CD33⁺ cells with small size and low side scatter) recovered from long-term engrafted mice from experiment no. 6 were deposited by ACDU into 96-well plates. Human granulocyte-macrophage clones were grown individually in methylcellulose medium with 0.9 mg/mL G418. Human T lymphocytes were expanded individually in medium containing rHuL-2 + PHA and irradiated feeders, then tested for the presence of the neo gene by PCR. Clonal integration analysis by inverse PCR was performed on the total marrow sample, and on each neo-positive human clone recovered from each mouse.

 Abbreviation: NA, not applicable; noneo-marked cells were detected in the marrow of the mouse, so further analyses were not done.

A total of 3 × 10⁵ bnx/hu bone marrow cells were plated ± G418 (0.9 mg/mL active) per sample.

A dash indicates that single-cell cloning was not performed.