Human Hematopoietic Lineages Recovered From the Bone Marrow of bnx Mice 6 to 12 Months Posttransplantation
| Mouse No. . | Transduction Conditions . | Months Engrafted . | % hu CD45+ . | % hu Cells of Each Lineage in bnx BM . | ||||
|---|---|---|---|---|---|---|---|---|
| BM . | spl . | CD4 . | CD8 . | CD33 . | CD19 . | |||
| 1A1 | BSA-FL | 8.5 | — | — | — | — | — | — |
| 1A2 | BSA-FL | 8.5 | 0.3 | — | — | — | — | — |
| 2A1 | BSA-FL | 10 | — | — | — | — | — | — |
| 2A2 | BSA-FL | 10 | — | — | — | — | — | — |
| 2A3 | BSA-FL | 10 | — | — | — | — | — | — |
| 5A1 | BSA-FL | 12 | — | — | — | — | — | — |
| 5A2 | BSA-FL | 12 | — | — | — | — | — | — |
| 5A3 | BSA-FL | 12 | — | — | — | — | — | — |
| 6A1 | BSA-FL | 6 | 0.2 | — | — | — | — | — |
| 6A2 | BSA-FL | 6 | — | — | — | — | — | — |
| 6A3 | BSA-FL | 6 | — | — | — | — | — | — |
| 4B1 | BSA + FL | 12 | 2.0 | — | 0.4 | 0.6 | 0.9 | — |
| 4B2 | BSA + FL | 12 | — | — | — | — | — | — |
| 4B3 | BSA + FL | 12 | 3.8 | — | 0.8 | 1.5 | 1.1 | — |
| 5B1 | BSA + FL | 12 | — | — | — | — | — | — |
| 6B1 | BSA + FL | 6 | — | 4.4 | — | — | — | — |
| 6B2 | BSA + FL | 6 | 0.2 | 0.2 | 0.1 | 0.1 | — | — |
| 6B3 | BSA + FL | 6 | 0.3 | 4.4 | 0.1 | 0.2 | — | — |
| 1C1 | Stroma-FL | 8.5 | 4.1 | — | 1.1 | 1.7 | 1.4 | — |
| 1C2 | Stroma-FL | 8.5 | 0.9 | — | 0.2 | 0.4 | 0.3 | — |
| 1C3 | Stroma-FL | 8.5 | 2.5 | — | 0.7 | 1.1 | 0.7 | — |
| 2C1 | Stroma-FL | 10 | 1.4 | — | 0.3 | 0.7 | 0.4 | — |
| 2C2 | Stroma-FL | 10 | 5.3 | — | 1.2 | 2.5 | 1.6 | — |
| 2C3 | Stroma-FL | 10 | — | — | — | — | — | — |
| 5C1 | Stroma-FL | 12 | 3.2 | — | 0.8 | 1.3 | 0.9 | — |
| 6C1 | Stroma-FL | 6 | 3.6 | — | 0.9 | 1.7 | 0.7 | — |
| 6C2 | Stroma-FL | 6 | 1.6 | 3.5 | 0.4 | 0.6 | 0.5 | — |
| 6C3 | Stroma-FL | 6 | 8.3 | 5.0 | 1.6 | 2.8 | 3.9 | — |
| 4D1 | Stroma + FL | 12 | 7.9 | — | 2.0 | 2.5 | 3.4 | — |
| 4D2 | Stroma + FL | 12 | 5.0 | — | 1.2 | 1.9 | 1.8 | — |
| 4D3 | Stroma + FL | 12 | 2.5 | — | 0.6 | 1.0 | 0.9 | — |
| 5D1 | Stroma + FL | 12 | 1.3 | — | 0.4 | 0.5 | 0.4 | — |
| 5D2 | Stroma + FL | 12 | 1.2 | — | 0.3 | 0.5 | 0.3 | — |
| 6D1 | Stroma + FL | 6 | 6.3 | 1.6 | 1.2 | 2.8 | 2.3 | — |
| 6D2 | Stroma + FL | 6 | 3.4 | 0.9 | 0.8 | 1.3 | 1.1 | — |
| 6D3 | Stroma + FL | 6 | 1.2 | — | 0.2 | 0.6 | 0.4 | — |
| 1E1 | FN-FL | 8.5 | 7.8 | 1.4 | 2.7 | 3.2 | 1.9 | — |
| 1E2 | FN-FL | 8.5 | 4.6 | — | 1.2 | 1.8 | 1.5 | — |
| 1E3 | FN-FL | 8.5 | — | — | — | — | — | — |
| 2E1 | FN-FL | 10 | 6.9 | — | 2.6 | 3.2 | 1.1 | — |
| 2E2 | FN-FL | 10 | 5.2 | — | 1.1 | 1.9 | 2.0 | — |
| 5E1 | FN-FL | 12 | 1.7 | — | 0.4 | 0.6 | 0.7 | — |
| 5E2 | FN-FL | 12 | 1.0 | — | 0.2 | 0.5 | 0.3 | — |
| 5E3 | FN-FL | 12 | 1.3 | — | 0.4 | 0.6 | 0.3 | — |
| 6E1 | FN-FL | 6 | 2.1 | — | 0.5 | 0.9 | 0.6 | — |
| 6E2 | FN-FL | 6 | 2.4 | — | 0.8 | 0.9 | 0.7 | — |
| 6E3 | FN-FL | 6 | 2.2 | — | 0.6 | 0.8 | 0.8 | — |
| 4F1 | FN + FL | 12 | 3.0 | 0.8 | 0.7 | 1.3 | 0.9 | — |
| 4F2 | FN + FL | 12 | 7.7 | — | 2.2 | 2.5 | 3.0 | — |
| 4F3 | FN + FL | 12 | 11.6 | 2.3 | 3.8 | 5.1 | 2.7 | — |
| 5F1 | FN + FL | 12 | 6.1 | — | 2.0 | 2.9 | 1.2 | — |
| 5F2 | FN + FL | 12 | 2.3 | — | 0.5 | 1.1 | 0.7 | — |
| 6F1 | FN + FL | 6 | 1.6 | — | 0.4 | 0.7 | 0.3 | — |
| 6F2 | FN + FL | 6 | 4.0 | 7.4 | 1.1 | 1.6 | 1.3 | — |
| 6F3 | FN + FL | 6 | 5.1 | 2.2 | 1.5 | 2.4 | 1.2 | — |
| Mouse No. . | Transduction Conditions . | Months Engrafted . | % hu CD45+ . | % hu Cells of Each Lineage in bnx BM . | ||||
|---|---|---|---|---|---|---|---|---|
| BM . | spl . | CD4 . | CD8 . | CD33 . | CD19 . | |||
| 1A1 | BSA-FL | 8.5 | — | — | — | — | — | — |
| 1A2 | BSA-FL | 8.5 | 0.3 | — | — | — | — | — |
| 2A1 | BSA-FL | 10 | — | — | — | — | — | — |
| 2A2 | BSA-FL | 10 | — | — | — | — | — | — |
| 2A3 | BSA-FL | 10 | — | — | — | — | — | — |
| 5A1 | BSA-FL | 12 | — | — | — | — | — | — |
| 5A2 | BSA-FL | 12 | — | — | — | — | — | — |
| 5A3 | BSA-FL | 12 | — | — | — | — | — | — |
| 6A1 | BSA-FL | 6 | 0.2 | — | — | — | — | — |
| 6A2 | BSA-FL | 6 | — | — | — | — | — | — |
| 6A3 | BSA-FL | 6 | — | — | — | — | — | — |
| 4B1 | BSA + FL | 12 | 2.0 | — | 0.4 | 0.6 | 0.9 | — |
| 4B2 | BSA + FL | 12 | — | — | — | — | — | — |
| 4B3 | BSA + FL | 12 | 3.8 | — | 0.8 | 1.5 | 1.1 | — |
| 5B1 | BSA + FL | 12 | — | — | — | — | — | — |
| 6B1 | BSA + FL | 6 | — | 4.4 | — | — | — | — |
| 6B2 | BSA + FL | 6 | 0.2 | 0.2 | 0.1 | 0.1 | — | — |
| 6B3 | BSA + FL | 6 | 0.3 | 4.4 | 0.1 | 0.2 | — | — |
| 1C1 | Stroma-FL | 8.5 | 4.1 | — | 1.1 | 1.7 | 1.4 | — |
| 1C2 | Stroma-FL | 8.5 | 0.9 | — | 0.2 | 0.4 | 0.3 | — |
| 1C3 | Stroma-FL | 8.5 | 2.5 | — | 0.7 | 1.1 | 0.7 | — |
| 2C1 | Stroma-FL | 10 | 1.4 | — | 0.3 | 0.7 | 0.4 | — |
| 2C2 | Stroma-FL | 10 | 5.3 | — | 1.2 | 2.5 | 1.6 | — |
| 2C3 | Stroma-FL | 10 | — | — | — | — | — | — |
| 5C1 | Stroma-FL | 12 | 3.2 | — | 0.8 | 1.3 | 0.9 | — |
| 6C1 | Stroma-FL | 6 | 3.6 | — | 0.9 | 1.7 | 0.7 | — |
| 6C2 | Stroma-FL | 6 | 1.6 | 3.5 | 0.4 | 0.6 | 0.5 | — |
| 6C3 | Stroma-FL | 6 | 8.3 | 5.0 | 1.6 | 2.8 | 3.9 | — |
| 4D1 | Stroma + FL | 12 | 7.9 | — | 2.0 | 2.5 | 3.4 | — |
| 4D2 | Stroma + FL | 12 | 5.0 | — | 1.2 | 1.9 | 1.8 | — |
| 4D3 | Stroma + FL | 12 | 2.5 | — | 0.6 | 1.0 | 0.9 | — |
| 5D1 | Stroma + FL | 12 | 1.3 | — | 0.4 | 0.5 | 0.4 | — |
| 5D2 | Stroma + FL | 12 | 1.2 | — | 0.3 | 0.5 | 0.3 | — |
| 6D1 | Stroma + FL | 6 | 6.3 | 1.6 | 1.2 | 2.8 | 2.3 | — |
| 6D2 | Stroma + FL | 6 | 3.4 | 0.9 | 0.8 | 1.3 | 1.1 | — |
| 6D3 | Stroma + FL | 6 | 1.2 | — | 0.2 | 0.6 | 0.4 | — |
| 1E1 | FN-FL | 8.5 | 7.8 | 1.4 | 2.7 | 3.2 | 1.9 | — |
| 1E2 | FN-FL | 8.5 | 4.6 | — | 1.2 | 1.8 | 1.5 | — |
| 1E3 | FN-FL | 8.5 | — | — | — | — | — | — |
| 2E1 | FN-FL | 10 | 6.9 | — | 2.6 | 3.2 | 1.1 | — |
| 2E2 | FN-FL | 10 | 5.2 | — | 1.1 | 1.9 | 2.0 | — |
| 5E1 | FN-FL | 12 | 1.7 | — | 0.4 | 0.6 | 0.7 | — |
| 5E2 | FN-FL | 12 | 1.0 | — | 0.2 | 0.5 | 0.3 | — |
| 5E3 | FN-FL | 12 | 1.3 | — | 0.4 | 0.6 | 0.3 | — |
| 6E1 | FN-FL | 6 | 2.1 | — | 0.5 | 0.9 | 0.6 | — |
| 6E2 | FN-FL | 6 | 2.4 | — | 0.8 | 0.9 | 0.7 | — |
| 6E3 | FN-FL | 6 | 2.2 | — | 0.6 | 0.8 | 0.8 | — |
| 4F1 | FN + FL | 12 | 3.0 | 0.8 | 0.7 | 1.3 | 0.9 | — |
| 4F2 | FN + FL | 12 | 7.7 | — | 2.2 | 2.5 | 3.0 | — |
| 4F3 | FN + FL | 12 | 11.6 | 2.3 | 3.8 | 5.1 | 2.7 | — |
| 5F1 | FN + FL | 12 | 6.1 | — | 2.0 | 2.9 | 1.2 | — |
| 5F2 | FN + FL | 12 | 2.3 | — | 0.5 | 1.1 | 0.7 | — |
| 6F1 | FN + FL | 6 | 1.6 | — | 0.4 | 0.7 | 0.3 | — |
| 6F2 | FN + FL | 6 | 4.0 | 7.4 | 1.1 | 1.6 | 1.3 | — |
| 6F3 | FN + FL | 6 | 5.1 | 2.2 | 1.5 | 2.4 | 1.2 | — |
Bone marrow and spleen samples were recovered from bnx mice 6 to 12 months after cotransplantation of transduced human hematopoietic cells and IL-3–producing stromal cells. The percentage of human CD45+ cells in the marrow (BM) and spleen (spl) were determined by FACS. In addition, marrow samples were tested for the percentages of human T, B, and myeloid cells that they contained. The percentages of human cells that were positive for each lineage marker by FACS analysis of the ungated bone marrow cells are shown. A dash indicates that no human hematopoietic cells were detected in that sample. In each experiment, normal human peripheral blood and age-matched, nontransplanted bnx mice were analyzed concurrently to verify the species-specificity of the monoclonal antibodies.