Effect of Different Cytokine Combinations on Cell Yield and T-Cell Immunophenotype Acquisition by CD34+ Cells in Fetal Thymic Organ-Cultured Lobes
| Cytokines Added . | Cell Expansion . | Differentiation Stages (%) . | ||||
|---|---|---|---|---|---|---|
| ISP . | DP . | SP CD4 . | SP CD8 . | CD56 . | ||
| SCF + Flt-3L + Il-7 | ×2 | 54 ± 30 | 4 ± 3 | 3 ± 2 | 0 | 3 ± 2 |
| SCF + Flt-3L + Il-7 + Il-1α | ×6-7 | 50 ± 50 | 50 ± 50 | 0 | 0 | 0 |
| SCF + Flt-3L + Il-7 + Il-1α + Il-15 | ×5-6 | 35 ± 20 | 10 ± 5 | 10 ± 5 | 2 ± 2 | 29 ± 15 |
| Cytokines Added . | Cell Expansion . | Differentiation Stages (%) . | ||||
|---|---|---|---|---|---|---|
| ISP . | DP . | SP CD4 . | SP CD8 . | CD56 . | ||
| SCF + Flt-3L + Il-7 | ×2 | 54 ± 30 | 4 ± 3 | 3 ± 2 | 0 | 3 ± 2 |
| SCF + Flt-3L + Il-7 + Il-1α | ×6-7 | 50 ± 50 | 50 ± 50 | 0 | 0 | 0 |
| SCF + Flt-3L + Il-7 + Il-1α + Il-15 | ×5-6 | 35 ± 20 | 10 ± 5 | 10 ± 5 | 2 ± 2 | 29 ± 15 |
Numbers represent mean ± SD (4 experiments).
Abbreviations: ISP, immature single positive; DP, double positive; SP, single positive.