Percentage of CD45+ Cells Detected in the BM of NOD/SCID Mice Injected With Unsorted AML Peripheral Blood Cells
Patient No. . | 5 × 106 . | No. of Cells Injected3-150 . | No. of Positive Colonies by FISH . | |||
---|---|---|---|---|---|---|
106 . | 5 × 105 . | 105 . | ≤5 × 104 . | |||
2 | 92 | 57 ± 3 | 2 | 0 | 0 | 7/8 |
3 | 21 | 15 | ND | ND | ND | 6/6 |
4 | 30 ± 10 | 42 | 5 | 0 | 4/123-151 | |
5 | 3 | 0 | ND | ND | ND | ND |
8 | 95 | 97 ± 0.6 | 92 | 66 | 26 | 26/26 |
9 | 2 | 0.2 | ND | ND | ND | 10/10 |
10 | 0.1 | 0 | 0 | 0 | ND | |
12 | ND | 0.2 | 0 | 0 | ND | |
13 | 0.14 | 0 | 0 | 0 | ND | |
15 | 30 ± 6 | 9 | 1 | 0 | ND | |
17 | 13 | 11 | 8 | 0 | ND | |
18 | 0 | 0 | ND | ND | ND |
Patient No. . | 5 × 106 . | No. of Cells Injected3-150 . | No. of Positive Colonies by FISH . | |||
---|---|---|---|---|---|---|
106 . | 5 × 105 . | 105 . | ≤5 × 104 . | |||
2 | 92 | 57 ± 3 | 2 | 0 | 0 | 7/8 |
3 | 21 | 15 | ND | ND | ND | 6/6 |
4 | 30 ± 10 | 42 | 5 | 0 | 4/123-151 | |
5 | 3 | 0 | ND | ND | ND | ND |
8 | 95 | 97 ± 0.6 | 92 | 66 | 26 | 26/26 |
9 | 2 | 0.2 | ND | ND | ND | 10/10 |
10 | 0.1 | 0 | 0 | 0 | ND | |
12 | ND | 0.2 | 0 | 0 | ND | |
13 | 0.14 | 0 | 0 | 0 | ND | |
15 | 30 ± 6 | 9 | 1 | 0 | ND | |
17 | 13 | 11 | 8 | 0 | ND | |
18 | 0 | 0 | ND | ND | ND |
Abbreviation: ND, not determined.
In four of the cases shown, the values represent the mean ± SEM % of CD45+ cells in femoral marrow of at least 3 NOD/SCID mice. All other values represent the result from a single mouse injected with the number of cells indicated.
Of the remaining 8 colonies, 5 were undetermined and 3 were negative.