Comparison of Total Blood Vessels With Neo-Stained and Cross-Linked, Fibrin-Stained Pulmonary Blood Vessels
| Lumenal Area (μm2) . | Frequency ( f ) . | Cumulative Relative f (%) . | ||||
|---|---|---|---|---|---|---|
| Total . | Neo . | FB . | Total . | Neo . | FB . | |
| 1-10 | 84 | 18 | 1 | 22 | 14 | 5 |
| 11-50 | 195 | 50 | 1 | 74 | 53 | 10 |
| 51-100 | 51 | 22 | 1 | 88 | 71 | 15 |
| 101-250 | 26 | 16 | 8 | 94 | 84 | 55 |
| 251-500 | 10 | 12 | 3 | 97 | 93 | 70 |
| 501-1,000 | 8 | 5 | 4 | 99 | 98 | 90 |
| 1,001-1,500 | 3 | 3 | 2 | 100 | 100 | 100 |
| n | 377 | 126 | 20 | |||
| Lumenal Area (μm2) . | Frequency ( f ) . | Cumulative Relative f (%) . | ||||
|---|---|---|---|---|---|---|
| Total . | Neo . | FB . | Total . | Neo . | FB . | |
| 1-10 | 84 | 18 | 1 | 22 | 14 | 5 |
| 11-50 | 195 | 50 | 1 | 74 | 53 | 10 |
| 51-100 | 51 | 22 | 1 | 88 | 71 | 15 |
| 101-250 | 26 | 16 | 8 | 94 | 84 | 55 |
| 251-500 | 10 | 12 | 3 | 97 | 93 | 70 |
| 501-1,000 | 8 | 5 | 4 | 99 | 98 | 90 |
| 1,001-1,500 | 3 | 3 | 2 | 100 | 100 | 100 |
| n | 377 | 126 | 20 | |||
Sequential lung sections were stained for H&E, neo- or cross-linked fibrin, and the lumenal area of positive vessels was traced electronically. The distribution of positive vessels from six microscope fields per condition is displayed as the frequency and the percent cumulative frequency. The correlation coefficient = 0.986 for the neo-positive vessels and corresponding H&E-stained vessels from a single microscope field of two sequential sections.