Alteration of Methylation Status and MDR1Expression During the Clinical Course in Two AML Patients
| Status . | Methylation Status3-150 . | MDR1 Gene Expression3-151 . | Clinical Outcome . |
|---|---|---|---|
| Pt. 13 | |||
| Primary diagnosis | Hyper (34%) | Negative (1.9) | CR |
| Relapse | Hypo (17%) | Positive (4.9) | Refractory |
| Pt. 14 | |||
| Primary diagnosis | Hyper (29%) | Negative (2.8) | CR |
| First relapse | Hyper (34%) | Negative (2.0) | CR |
| Second relapse | Hypo (22%) | Positive (4.4) | Refractory |
| Status . | Methylation Status3-150 . | MDR1 Gene Expression3-151 . | Clinical Outcome . |
|---|---|---|---|
| Pt. 13 | |||
| Primary diagnosis | Hyper (34%) | Negative (1.9) | CR |
| Relapse | Hypo (17%) | Positive (4.9) | Refractory |
| Pt. 14 | |||
| Primary diagnosis | Hyper (29%) | Negative (2.8) | CR |
| First relapse | Hyper (34%) | Negative (2.0) | CR |
| Second relapse | Hypo (22%) | Positive (4.4) | Refractory |
Methylation status was analyzed by PCR (Fig 6) and represented as % valued in parenthesis. Hypo and hyper indicate hypomethylation and hypermethylation status defined as described in Results, respectively.
MDR1 expression was determined as described in the legend to Fig 4. Negative means <3.0, and positive means >3.0.