Table 2. Effect of Increasing... | American Society of Hematology

Table 2.

Effect of Increasing Extracellular Ca²⁺ on Dense Cell Formation by O-D Cycling

SS Patient	Ca²⁺ (mmol/L)	% Cells After 22 Hours of O-D Cycling
SS Patient	Ca²⁺ (mmol/L)	LD	ND	ID	HD
N.M.	0.5	7	16	46	31
	2.0	3	7	38	52
A.O.	0.5	2	39	43	16
	2.0	<1	15	48	37

SS Patient	Ca²⁺ (mmol/L)	% Cells After 22 Hours of O-D Cycling
SS Patient	Ca²⁺ (mmol/L)	LD	ND	ID	HD
N.M.	0.5	7	16	46	31
	2.0	3	7	38	52
A.O.	0.5	2	39	43	16
	2.0	<1	15	48	37

SS-ND cells were O-D cycled for 22 hours at pH 6.8 in buffer containing physiologic levels of Cl⁻, after which the cells were separated using continuous density gradients and quantitated, as in Fig 1. LD, <1.080 g/mL; ND, 1.080 to 1.090 g/mL; ID, 1.090 to 1.114 g/mL; HD, >1.114 g/mL.

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