Table 1. Effect of Replacing... | American Society of Hematology

Table 1.

Effect of Replacing Extracellular Ca²⁺With EGTA on Dense Cell Formation

Condition (n = 4)	% Cells After 22 Hours of O-D Cycling
Condition (n = 4)	LD	ND	ID	HD
0.5 mmol/L Ca²⁺	6 ± 2	32 ± 24	38 ± 26	24 ± 8
0.5 mmol/L EGTA	7 ± 3	33 ± 20	51 ± 13	9 ± 8
				p < .043v Ca²⁺

Condition (n = 4)	% Cells After 22 Hours of O-D Cycling
Condition (n = 4)	LD	ND	ID	HD
0.5 mmol/L Ca²⁺	6 ± 2	32 ± 24	38 ± 26	24 ± 8
0.5 mmol/L EGTA	7 ± 3	33 ± 20	51 ± 13	9 ± 8
				p < .043v Ca²⁺

SS-ND cells were O-D cycled for 22 hours at pH 6.8 in buffer containing physiologic levels of Cl⁻, after which the cells were separated using continuous density gradients and quantitated, as in Fig 1. LD, <1.080 g/mL; ND, 1.080 to 1.090 g/mL; ID, 1.090 to 1.114 g/mL; HD, >1.114 g/mL.

View Large

This Feature Is Available To Subscribers Only