RT-PCR Analysis of ▵5, 6 Mutant Mice
| . | . | Δ5, 6 H . | Δ5, 6 ΔH . |
|---|---|---|---|
| Ey | dpc 10.5 | 0.96 ± 0.05 (2) | 0.98 ± 0.05 (4) |
| βh1 | dpc 10.5 | 0.83 ± 0.01 (2) | 0.90 ± 0.03 (4) |
| Adult β | dpc 15.5 | 0.97 ± 0.09 (4) | 0.87 ± 0.05 (3) |
| Adult β | Adult | 0.91 ± 0.03 (3) | 1.00 ± 0.08 (5) |
| . | . | Δ5, 6 H . | Δ5, 6 ΔH . |
|---|---|---|---|
| Ey | dpc 10.5 | 0.96 ± 0.05 (2) | 0.98 ± 0.05 (4) |
| βh1 | dpc 10.5 | 0.83 ± 0.01 (2) | 0.90 ± 0.03 (4) |
| Adult β | dpc 15.5 | 0.97 ± 0.09 (4) | 0.87 ± 0.05 (3) |
| Adult β | Adult | 0.91 ± 0.03 (3) | 1.00 ± 0.08 (5) |
Ratio of expression from a mutant D allele to that of a wild-type S allele measured in heterozygotic animals followed by the standard deviation. The number in parentheses is the number of animals analyzed. RNA from erythroid tissue was isolated and analyzed by RT-PCR. For each sample two independent RT reactions were generated and each was analyzed in duplicate or triplicate.