MIP-1 Receptor Downregulation in Cultured CD34+ Cells Is Partially Reversed by TNF-
| Experiment No. . | Time (h) . | ↓ + TNFα . | |||
|---|---|---|---|---|---|
| 0 . | 4 . | 10 . | 24 . | 36 . | |
| 1 | 71.0 | 119.0 | 95.6 | 16.1 | 79.5 |
| 2 | 190.6 | 250.0 | 45.4 | 11.5 | 52.0 |
| Experiment No. . | Time (h) . | ↓ + TNFα . | |||
|---|---|---|---|---|---|
| 0 . | 4 . | 10 . | 24 . | 36 . | |
| 1 | 71.0 | 119.0 | 95.6 | 16.1 | 79.5 |
| 2 | 190.6 | 250.0 | 45.4 | 11.5 | 52.0 |
LP CD34+ cells were cultured in serum-supplemented medium in the absence of TNF-α. MIP-1α receptor expression on CD34+ cells was analyzed flow-cytometrically before and after the addition of TNF-α (2 ng/mL at 24 hours). Data are presented as mean fluorescence intensity values; two separate experiments are shown.