Effect of E Subunit Mutations on Secretion of Fib420 in COS Cells Transfected With E, β, and γ cDNAs
| Mutation . | Fib420 Secretion . |
|---|---|
| Wild-type | + |
| 28-S and 36-S | + |
| 442-S and 472-S | + |
| 613-S | − |
| 644-S | − |
| 780-S | − |
| 793-S | − |
| 613-S and 644-S | + |
| 780-S and 793-S | − |
| 780-S and 793-A | − |
| 613-S and 780-S | − |
| 644-S and 793-S | − |
| 613-S, 644-S, 780-S, and 793-S | − |
| 667-Q | + |
| 812-Q | + |
| Mutation . | Fib420 Secretion . |
|---|---|
| Wild-type | + |
| 28-S and 36-S | + |
| 442-S and 472-S | + |
| 613-S | − |
| 644-S | − |
| 780-S | − |
| 793-S | − |
| 613-S and 644-S | + |
| 780-S and 793-S | − |
| 780-S and 793-A | − |
| 613-S and 780-S | − |
| 644-S and 793-S | − |
| 613-S, 644-S, 780-S, and 793-S | − |
| 667-Q | + |
| 812-Q | + |
Cys → Ser (S); Cys → Ala (A); Asn → Gln (Q).