Effect of Accutin (0.2 μmol/L) on Anti-Integrin MoAbs Binding to HUVEC
| Pretreatment MoAb . | Fluorescence Intensity . | % Positively Staining Cells . | ||||
|---|---|---|---|---|---|---|
| PBS . | Accutin 0.2 μmol/L . | P Value . | PBS . | Accutin 0.2 μmol/L . | P Value . | |
| Control IgG | 19.11 ± 0.64 | 15.3 ± 6.87 | .42 | 7.87 ± 0.69 | 6.87 ± 1.30 | .48 |
| (n = 11) | (n = 4) | (n = 11) | (n = 4) | |||
| Anti-α2 integrin | 98.94 ± 7.75 | 83.23 ± 12.96 | .31 | 88.42 ± 1.93 | 77.57 ± 7.86 | .07 |
| (n = 7) | (n = 3) | (n = 7) | (n = 3) | |||
| Anti-α3 integrin | 34.66 ± 1.51 | 36.08 ± 1.76 | .61 | 32.44 ± 2.21 | 36.34 ± 5.16 | .16 |
| (n = 11) | (n = 4) | (n = 11) | (n = 4) | |||
| Anti-α4 integrin | 23.22 ± 3.24 | ND | — | 9.26 ± 2.40 | ND | — |
| (n = 4) | (n = 4) | |||||
| Anti-α5 integrin | 72.90 ± 3.92 | 87.16 ± 10.62 | .18 | 80.69 ± 3.92 | 77.82 ± 6.17 | .68 |
| (n = 11) | (n = 3) | (n = 11) | (n = 3) | |||
| 7E3 | 68.97 ± 3.36 | 34.5 ± 1.94 | <.001 | 93.84 ± 2.91 | 21.84 ± 2.88 | <.001 |
| (n = 9) | (n = 4) | (n = 9) | (n = 4) | |||
| Pretreatment MoAb . | Fluorescence Intensity . | % Positively Staining Cells . | ||||
|---|---|---|---|---|---|---|
| PBS . | Accutin 0.2 μmol/L . | P Value . | PBS . | Accutin 0.2 μmol/L . | P Value . | |
| Control IgG | 19.11 ± 0.64 | 15.3 ± 6.87 | .42 | 7.87 ± 0.69 | 6.87 ± 1.30 | .48 |
| (n = 11) | (n = 4) | (n = 11) | (n = 4) | |||
| Anti-α2 integrin | 98.94 ± 7.75 | 83.23 ± 12.96 | .31 | 88.42 ± 1.93 | 77.57 ± 7.86 | .07 |
| (n = 7) | (n = 3) | (n = 7) | (n = 3) | |||
| Anti-α3 integrin | 34.66 ± 1.51 | 36.08 ± 1.76 | .61 | 32.44 ± 2.21 | 36.34 ± 5.16 | .16 |
| (n = 11) | (n = 4) | (n = 11) | (n = 4) | |||
| Anti-α4 integrin | 23.22 ± 3.24 | ND | — | 9.26 ± 2.40 | ND | — |
| (n = 4) | (n = 4) | |||||
| Anti-α5 integrin | 72.90 ± 3.92 | 87.16 ± 10.62 | .18 | 80.69 ± 3.92 | 77.82 ± 6.17 | .68 |
| (n = 11) | (n = 3) | (n = 11) | (n = 3) | |||
| 7E3 | 68.97 ± 3.36 | 34.5 ± 1.94 | <.001 | 93.84 ± 2.91 | 21.84 ± 2.88 | <.001 |
| (n = 9) | (n = 4) | (n = 9) | (n = 4) | |||
PBS or accutin (0.2 μmol/L) was preincubated with HUVECs, and the binding reaction of various anti-integrin MoAbs to HUVECs was analyzed separately as described in Materials and Methods. All antibodies were used at a 1:50 dilution. Control samples contain HUVECs in the presence of mouse nonimmune IgG. Values are presented as mean ± SEM. P value was determined by paired Student’st-test.
Abbreviation: ND, not determined.