Inhibition of Cellular Growth and Induction of Benzidine-Positive Cells by Chemical Inducers in the Cell Lines Used as Sources of RNA for RNase Protection Assays
| Inducer . | Concentration . | Friend MEL-PC4 . | Ba/F3 . | B6SUtA . | |||
|---|---|---|---|---|---|---|---|
| CD × 10−6 (% GI) . | % BP . | CD × 10−6 (% GI) . | % BP . | CD × 10−6 (% GI) . | % BP . | ||
| None | — | 3.60 (0) | 0 | 1.95 (0) | 0 | 1.04 (0) | 4 |
| DMSO | 128 mmol/L | — | — | 0.39 (34) | 4 | ||
| 192 mmol/L | 2.12 (12) | 41 | 0.60 (30) | 0 | 0.16 (65) | 4 | |
| 256 mmol/L | 0.81 (33) | 65 | 0.12 (71) | 0 | — | ||
| SB | 0.5 mmol/L | — | — | 0.45 (29) | 25 | ||
| 1.0 mmol/L | — | 0.62 (30) | 30 | 0.12 (77) | 71 | ||
| 1.5 mmol/L | 1.05 (26) | 5 | 0.17 (62) | 34 | — | ||
| 2.0 mmol/L | 0.40 (49) | 15 | — | — | |||
| TSA | 10 nmol/L | — | — | 0.44 (30) | 36 | ||
| 15 nmol/L | — | 0.43 (39) | 32 | — | |||
| 20 nmol/L | 1.43 (22) | 4 | 0.17 (62) | 30 | 0.19 (59) | 75 | |
| 25 nmol/L | 0.70 (37) | 13 | — | — | |||
| D2 | 0.05 mmol/L | — | — | 0.51 (25) | 5 | ||
| 0.10 mmol/L | 1.89 (14) | 39 | 0.87 (21) | 3 | 0.16 (65) | 37 | |
| 0.15 mmol/L | 0.71 (36) | 72 | 0.11 (75) | 0 | — | ||
| H | 0.05 mmol/L | 3.61 (0) | 14 | 1.95 (0) | 17 | 1.04 (0) | 48 |
| 0.10 mmol/L | 3.44 (1) | 18 | 1.83 (2) | 24 | 0.93 (4) | 59 | |
| Inducer . | Concentration . | Friend MEL-PC4 . | Ba/F3 . | B6SUtA . | |||
|---|---|---|---|---|---|---|---|
| CD × 10−6 (% GI) . | % BP . | CD × 10−6 (% GI) . | % BP . | CD × 10−6 (% GI) . | % BP . | ||
| None | — | 3.60 (0) | 0 | 1.95 (0) | 0 | 1.04 (0) | 4 |
| DMSO | 128 mmol/L | — | — | 0.39 (34) | 4 | ||
| 192 mmol/L | 2.12 (12) | 41 | 0.60 (30) | 0 | 0.16 (65) | 4 | |
| 256 mmol/L | 0.81 (33) | 65 | 0.12 (71) | 0 | — | ||
| SB | 0.5 mmol/L | — | — | 0.45 (29) | 25 | ||
| 1.0 mmol/L | — | 0.62 (30) | 30 | 0.12 (77) | 71 | ||
| 1.5 mmol/L | 1.05 (26) | 5 | 0.17 (62) | 34 | — | ||
| 2.0 mmol/L | 0.40 (49) | 15 | — | — | |||
| TSA | 10 nmol/L | — | — | 0.44 (30) | 36 | ||
| 15 nmol/L | — | 0.43 (39) | 32 | — | |||
| 20 nmol/L | 1.43 (22) | 4 | 0.17 (62) | 30 | 0.19 (59) | 75 | |
| 25 nmol/L | 0.70 (37) | 13 | — | — | |||
| D2 | 0.05 mmol/L | — | — | 0.51 (25) | 5 | ||
| 0.10 mmol/L | 1.89 (14) | 39 | 0.87 (21) | 3 | 0.16 (65) | 37 | |
| 0.15 mmol/L | 0.71 (36) | 72 | 0.11 (75) | 0 | — | ||
| H | 0.05 mmol/L | 3.61 (0) | 14 | 1.95 (0) | 17 | 1.04 (0) | 48 |
| 0.10 mmol/L | 3.44 (1) | 18 | 1.83 (2) | 24 | 0.93 (4) | 59 | |
Cells were exposed to chemical inducers at the indicated concentrations. At day 3, CD was measured and the percent GI was calculated by methodology described in Materials and Methods. The percent BP was determined as previously described.4
Abbreviations: DMSO, dimethylsulfoxide; SB, sodium butyrate; TSA, trichostatin A; D2, diazepam; H, hemin; CD, cell density; GI, growth inhibition; BP, benzidine-positive cells.