PCR and RACE Primers
| Exon . | Sense . | Antisense . | Sequence . |
|---|---|---|---|
| 1a | o134 | CTTGCCTGGCTATCACCAG | |
| 1 | o99 | CCGAGGATGCGACGCACCGCAG | |
| 3 | 5541 | CTGACTGAGACACATCAGCAGCAC | |
| 3 | 5760 | GGGGACTCTGCTTGATGCTAA | |
| 3 | 5540 | CATCTGGGCTGGATGGAATTTAG | |
| 4 | 5761 | CTCTTCCAGTGTTTGGACAAG | |
| 6 | o58 | AAGCCAAGTTCACCTTTCTCTATGTG | |
| 6 | o48 | CCTCAATTTCCCTGAAATTGGTT | |
| 7 | o103 | AAGAGCTGCTCAGGTCACAGTGG | |
| 10 | o143 | CTCCTTCTGCATCCTTAACTG | |
| 11 | o93 | CCTTACCATGCAAAAATGCGGAC | |
| 19 | o76 | GTGCAACTGCAAGCCCACAGATG | |
| 24 | o77 | GCCACACACGTCACAATGATGCC | |
| 24 | o80 | ATATCTAGAGTGGCGGTAACGCTGAGGAGTGA | |
| 24 | o83 | TCCTTGGCATCCGAAACCAG | |
| 24 | o82 | CCGTGGGACACAGGCATTTTAC | |
| Iμ | 5518 | CAGATCTGAAAGTGCTCTACTG | |
| Iμ | o52 | AGGATCCGGCAGCAGAAGCCACGCATCCCAGCTCTG | |
| Iμ | 5536 | AGCCCTTGTTAATGGACTTGGAGG | |
| JH1 | 5590 | CCCTGGTCACCGTCTCCTCA | |
| JH3 | 5592 | CAATGGTCACCGTCTCTTCA | |
| Cγ | o64 | TGTCCTTGGGTTTTGGGGGGAA | |
| Cα | o65 | TCTCTCAGGCCGGTCAGTGTGC | |
| Cμ | o132 | GAAGACGCTCACTTTGGGAGG | |
| RACE | 5051 | TAGGAATTCGATCTCGAGGCTTTTTTTTTTTTTTTTT(ACG) | |
| RACE | 5052 | TAGGAATTCGATCTCGAGGC |
| Exon . | Sense . | Antisense . | Sequence . |
|---|---|---|---|
| 1a | o134 | CTTGCCTGGCTATCACCAG | |
| 1 | o99 | CCGAGGATGCGACGCACCGCAG | |
| 3 | 5541 | CTGACTGAGACACATCAGCAGCAC | |
| 3 | 5760 | GGGGACTCTGCTTGATGCTAA | |
| 3 | 5540 | CATCTGGGCTGGATGGAATTTAG | |
| 4 | 5761 | CTCTTCCAGTGTTTGGACAAG | |
| 6 | o58 | AAGCCAAGTTCACCTTTCTCTATGTG | |
| 6 | o48 | CCTCAATTTCCCTGAAATTGGTT | |
| 7 | o103 | AAGAGCTGCTCAGGTCACAGTGG | |
| 10 | o143 | CTCCTTCTGCATCCTTAACTG | |
| 11 | o93 | CCTTACCATGCAAAAATGCGGAC | |
| 19 | o76 | GTGCAACTGCAAGCCCACAGATG | |
| 24 | o77 | GCCACACACGTCACAATGATGCC | |
| 24 | o80 | ATATCTAGAGTGGCGGTAACGCTGAGGAGTGA | |
| 24 | o83 | TCCTTGGCATCCGAAACCAG | |
| 24 | o82 | CCGTGGGACACAGGCATTTTAC | |
| Iμ | 5518 | CAGATCTGAAAGTGCTCTACTG | |
| Iμ | o52 | AGGATCCGGCAGCAGAAGCCACGCATCCCAGCTCTG | |
| Iμ | 5536 | AGCCCTTGTTAATGGACTTGGAGG | |
| JH1 | 5590 | CCCTGGTCACCGTCTCCTCA | |
| JH3 | 5592 | CAATGGTCACCGTCTCTTCA | |
| Cγ | o64 | TGTCCTTGGGTTTTGGGGGGAA | |
| Cα | o65 | TCTCTCAGGCCGGTCAGTGTGC | |
| Cμ | o132 | GAAGACGCTCACTTTGGGAGG | |
| RACE | 5051 | TAGGAATTCGATCTCGAGGCTTTTTTTTTTTTTTTTT(ACG) | |
| RACE | 5052 | TAGGAATTCGATCTCGAGGC |
The sequence of the primers used in a PCR assay to amplify probes for the Northern blot analysis and to screen the cDNA library, in RACE experiments, for sequencing analysis, and for amplification of hybrid transcripts are listed below in a 5′-3′ orientation.