PCR Primers
| 5′ Primers | |
| Pan VH-consensus primer (VH-FR1) | 5′-CAGGTGCAGCTGGTGGAGTCTGGG-3′ |
| VH3 (leader sequence) | 5′-GGGTCGACTCCATGGAGTTTGGGCTGAGC-3′ |
| VH6 (leader sequence) | 5′-GGGTCGACTGTCTCCTTCCTCATCTTCCT-3′ |
| Pan VH-FR3 | 5′-GGGTCGACACGGCCGTGTATTACTGT-3′ |
| 3′ Primers | |
| Cμ 15 | 5′-GACGAAGACGCTCACTTTGGG-3′ |
| Cγ 15 | 5′-GTGATTCACGTTGCAGGTGTAGGT-3′ |
| Cμ 5 | 5′-GGGAATTCCTGCTGATGTCAGAGTTGTT-3′ |
| Cγ 5 | 5′-GGGAATTCGCTGGTCAGGGCGCCTGAGTT-3′ |
| Cμ 2 | 5′-GGAATTCTCACAGGAGACG-3′ |
| Cγ 2 | 5′-GGAATTCCTGGAGCAGGGCGCC-3′ |
| Cμ 2-RC* | 5′-CGTCTCCTGTGAGAATTCC-3′ |
| 5′ Primers | |
| Pan VH-consensus primer (VH-FR1) | 5′-CAGGTGCAGCTGGTGGAGTCTGGG-3′ |
| VH3 (leader sequence) | 5′-GGGTCGACTCCATGGAGTTTGGGCTGAGC-3′ |
| VH6 (leader sequence) | 5′-GGGTCGACTGTCTCCTTCCTCATCTTCCT-3′ |
| Pan VH-FR3 | 5′-GGGTCGACACGGCCGTGTATTACTGT-3′ |
| 3′ Primers | |
| Cμ 15 | 5′-GACGAAGACGCTCACTTTGGG-3′ |
| Cγ 15 | 5′-GTGATTCACGTTGCAGGTGTAGGT-3′ |
| Cμ 5 | 5′-GGGAATTCCTGCTGATGTCAGAGTTGTT-3′ |
| Cγ 5 | 5′-GGGAATTCGCTGGTCAGGGCGCCTGAGTT-3′ |
| Cμ 2 | 5′-GGAATTCTCACAGGAGACG-3′ |
| Cγ 2 | 5′-GGAATTCCTGGAGCAGGGCGCC-3′ |
| Cμ 2-RC* | 5′-CGTCTCCTGTGAGAATTCC-3′ |
Abbreviation: FR, framework.
Complementary to the Cμ 2 primer sequence. This primer is used in combination with Cμ 15 to amplify constant region transcripts.