Cytokine Levels Present in Supernatants Derived From an Alloantigen-Stimulated Cell Line With Antiviral Activity
| . | Cytokine Levels (ng/mL) . | ||
|---|---|---|---|
| Day 2 . | Day 4 . | Day 7 . | |
| IFN-γ | 6.27 | 27.43 | 2.63 |
| IFN-α | 0.05 | 0.05 | 0.02 |
| TNF-α | 0.25 | 0.17 | 0.34 |
| IL-2 | 5.14 | 1.61 | 0.67 |
| IL-4 | 0.02 | 0.007 | 0.005 |
| IL-10 | 0.77 | 0.68 | 0.68 |
| IL-16 | 0.47 | 1.3 | 1.0 |
| MIP-1α | 9.85 | 7.45 | 5.41 |
| MIP-1β | 11.48 | 11.44 | 9.30 |
| RANTES | 9.52 | 1.25 | 1.63 |
| % inhibition of HIV replication | 99.7 | 95.6 | 98.5 |
| . | Cytokine Levels (ng/mL) . | ||
|---|---|---|---|
| Day 2 . | Day 4 . | Day 7 . | |
| IFN-γ | 6.27 | 27.43 | 2.63 |
| IFN-α | 0.05 | 0.05 | 0.02 |
| TNF-α | 0.25 | 0.17 | 0.34 |
| IL-2 | 5.14 | 1.61 | 0.67 |
| IL-4 | 0.02 | 0.007 | 0.005 |
| IL-10 | 0.77 | 0.68 | 0.68 |
| IL-16 | 0.47 | 1.3 | 1.0 |
| MIP-1α | 9.85 | 7.45 | 5.41 |
| MIP-1β | 11.48 | 11.44 | 9.30 |
| RANTES | 9.52 | 1.25 | 1.63 |
| % inhibition of HIV replication | 99.7 | 95.6 | 98.5 |
Supernatants from allostimulated cell lines were obtained at the indicated days after stimulation with allogeneic cells, as indicated in Materials and Methods. Cytokine levels in the supernatants were determined by ELISA. Results represent means of duplicates.