Effect of Supernatants Obtained From Several Types of Stimulation on HIV-1 p24 Antigen Production by Infected Lymphoblasts
| . | HIV-1 p24 Antigen (ng/mL) . |
|---|---|
| Control unstimulated supernatant | 66.9 ± 14.1 |
| Tetanus-stimulated supernatant | 124.3 ± 10.6 |
| Anti-CD3–stimulated supernatant | 46.7 ± 4.7 |
| Control alloantigen supernatant | 56.2 ± 38.3 |
| Alloantigen-stimulated supernatant | <2 |
| . | HIV-1 p24 Antigen (ng/mL) . |
|---|---|
| Control unstimulated supernatant | 66.9 ± 14.1 |
| Tetanus-stimulated supernatant | 124.3 ± 10.6 |
| Anti-CD3–stimulated supernatant | 46.7 ± 4.7 |
| Control alloantigen supernatant | 56.2 ± 38.3 |
| Alloantigen-stimulated supernatant | <2 |
The results represent average ± SD of two experiments performed in triplicate. PHA blasts were infected with HIV-1BZ167(172 TCID50/105 cells) and cultured in the presence of supernatants derived from alloantigen-stimulated cell lines, tetanus toxoid-, and anti-CD3–stimulated PBMC, as described in Materials and Methods. p24 antigen production was determined by ELISA 3 days post-infection. Control cultures for tetanus toxoid- or anti-CD3–stimulated supernatants were supernatants derived from unstimulated cells. Control cultures for alloantigen-stimulated cell line supernatant were culture media.