Clonotypic IgH CDR3 Sequences and Primers Used for PSA
| ....CDR2 PRIMER .................FR3 | CDR3............................. JH | ||
| 01. | CACCAAGGACTCCTCCAAAA//TGTGCTCAC | ... | AAACTTATCACTGGTTGGGACGGTAGTAGTTACTTTGACCAG.TGGGGC |
| 02. | TGGGAGTCATCTATCCTGGTG//TGTGCGACA | ... | CAACACTACTATGATAGTTATATTGACTTC.TGGGGC |
| 03. | AGACTCAATGAAGGGCCGAT//TGTGCGAGA | ... | GATACCTATTATTATGGTTCAGGGAGTTATTCATACTGGTACTTCGATCTC.TGGGGC |
| 04. | ACTTCTACGACAATGGCGAAAC//TGTGCGGGT | ... | GGCACCACGTCCTCCCAGGGTCAGAGGTTGGAACTC.TGGGGC |
| 05. | CACCATTCAGATTCCGTGAAG//TGTGCGAAG | ... | CTCGTGGTTGTGGCGGTGGAAGCTCTAACCCAT GATAATCTTGATATT.TGGGGC |
| 06. | ACCAACATACAATCCCTCCCTC//TGTGCGAGG | ... | GTCCCCATGAACTATGCTATAAGGGGAAACTTAGGT TCCATTGACTAC.TGGGGC |
| 07. | AGTGGGGACAGCAGTGAAAT//TGTGCGAGA | ... | GAATGGTCGTACTTCTATGAAAGTTATTGGTTA TTACCCTTTGACTTC.TGGGGC |
| 08. | CTTATGTCATATGATGAAGGC//TGTGCGAGA | ... | GACGGAAGCAGAGATGGCTACAACTCG GGTGTTTTTGATATC.TGGGGC |
| 09. | ACCATCTCCAGAGACAACGC//TGTGCGAGA | ... | GGGGATGGTTCGGGAGAGATCTTT CCTTACTACTACTATCACATGGACGTC.TGGGGC |
| 10. | ATGAGCGCTACAACCCATCT//TGTTCACAC | ... | ACGCGTTTCATGCCTGCGGATGTGAACAACTTCTTTGACTAC.TGGGGC |
| 11. | TGGCACTTATTTCAGATGATGG//TGTGCGCCA | ... | GTTCTTGCCAACTGGTTTCGCCCCTTTGACCAC.TGGGGC |
| 12. | GTGGTGGGACAACAGACTCC//TGTACCACA | ... | GCGTTCAGTGAGCCCTCCAGCGACTACTACACGATGGACTTC.TGGGGC |
| 13. | AGTACTGGCGCTGTCACAAA//TGTGCGACA | ... | GATCAAGATGACTACGGTGACTACGGGACCTTTAACTCC.TGGGGC |
| 14. | TGGAGTGGGTGGCTTCTTAT//TGTACGAGA | ... | GTAAATCCTTTCTATGAAGGTAGTCGTTATCCCATA TACTACTTTGGCTAC.TGGGGC |
| 15. | TCCTCCAGTGGAAGCACCTA//TGCGCCAGA | ... | GATCCCTCTGACTACTTTGACCTC.TGGGGC |
| 16. | TCATCCCTATCTTTGGCACA//TGTGCGACA | ... | GTAAATCCTTTCTATGAAGGTAGTCGTTATCCCATA TACTACTTTGGCTAC.TGGGGC |
| 17. | GCTGGAGTGGGTAGGTTTCA//TGTACTAGA | ... | GATAGGGAGGATACTGTAGTAGGAACAGTTACTATGGGCCGAATACCCACGGTT AAATACTACTACTCCACCACATGGACGTC.TGGGGC |
| 18. | TGGGTGGGAATCATCTATCC//TGTGCGAGA | ... | CATTATCACGGTTACCGATCGGACGTC.TGGGGC |
| 19. | CCATCAACTACATTGCTGACA//TGTGCGAAG | ... | GGCCATCGCCATTGTAATGGAGGTGGCTGCGCCGTCCTGGACCTC.TGGGGC |
| ....CDR2 PRIMER .................FR3 | CDR3............................. JH | ||
| 01. | CACCAAGGACTCCTCCAAAA//TGTGCTCAC | ... | AAACTTATCACTGGTTGGGACGGTAGTAGTTACTTTGACCAG.TGGGGC |
| 02. | TGGGAGTCATCTATCCTGGTG//TGTGCGACA | ... | CAACACTACTATGATAGTTATATTGACTTC.TGGGGC |
| 03. | AGACTCAATGAAGGGCCGAT//TGTGCGAGA | ... | GATACCTATTATTATGGTTCAGGGAGTTATTCATACTGGTACTTCGATCTC.TGGGGC |
| 04. | ACTTCTACGACAATGGCGAAAC//TGTGCGGGT | ... | GGCACCACGTCCTCCCAGGGTCAGAGGTTGGAACTC.TGGGGC |
| 05. | CACCATTCAGATTCCGTGAAG//TGTGCGAAG | ... | CTCGTGGTTGTGGCGGTGGAAGCTCTAACCCAT GATAATCTTGATATT.TGGGGC |
| 06. | ACCAACATACAATCCCTCCCTC//TGTGCGAGG | ... | GTCCCCATGAACTATGCTATAAGGGGAAACTTAGGT TCCATTGACTAC.TGGGGC |
| 07. | AGTGGGGACAGCAGTGAAAT//TGTGCGAGA | ... | GAATGGTCGTACTTCTATGAAAGTTATTGGTTA TTACCCTTTGACTTC.TGGGGC |
| 08. | CTTATGTCATATGATGAAGGC//TGTGCGAGA | ... | GACGGAAGCAGAGATGGCTACAACTCG GGTGTTTTTGATATC.TGGGGC |
| 09. | ACCATCTCCAGAGACAACGC//TGTGCGAGA | ... | GGGGATGGTTCGGGAGAGATCTTT CCTTACTACTACTATCACATGGACGTC.TGGGGC |
| 10. | ATGAGCGCTACAACCCATCT//TGTTCACAC | ... | ACGCGTTTCATGCCTGCGGATGTGAACAACTTCTTTGACTAC.TGGGGC |
| 11. | TGGCACTTATTTCAGATGATGG//TGTGCGCCA | ... | GTTCTTGCCAACTGGTTTCGCCCCTTTGACCAC.TGGGGC |
| 12. | GTGGTGGGACAACAGACTCC//TGTACCACA | ... | GCGTTCAGTGAGCCCTCCAGCGACTACTACACGATGGACTTC.TGGGGC |
| 13. | AGTACTGGCGCTGTCACAAA//TGTGCGACA | ... | GATCAAGATGACTACGGTGACTACGGGACCTTTAACTCC.TGGGGC |
| 14. | TGGAGTGGGTGGCTTCTTAT//TGTACGAGA | ... | GTAAATCCTTTCTATGAAGGTAGTCGTTATCCCATA TACTACTTTGGCTAC.TGGGGC |
| 15. | TCCTCCAGTGGAAGCACCTA//TGCGCCAGA | ... | GATCCCTCTGACTACTTTGACCTC.TGGGGC |
| 16. | TCATCCCTATCTTTGGCACA//TGTGCGACA | ... | GTAAATCCTTTCTATGAAGGTAGTCGTTATCCCATA TACTACTTTGGCTAC.TGGGGC |
| 17. | GCTGGAGTGGGTAGGTTTCA//TGTACTAGA | ... | GATAGGGAGGATACTGTAGTAGGAACAGTTACTATGGGCCGAATACCCACGGTT AAATACTACTACTCCACCACATGGACGTC.TGGGGC |
| 18. | TGGGTGGGAATCATCTATCC//TGTGCGAGA | ... | CATTATCACGGTTACCGATCGGACGTC.TGGGGC |
| 19. | CCATCAACTACATTGCTGACA//TGTGCGAAG | ... | GGCCATCGCCATTGTAATGGAGGTGGCTGCGCCGTCCTGGACCTC.TGGGGC |
The sequence of CDR2 chosen for PSA priming is indicated, followed by a break in the continuous VDJ sequence and then by the complete CDR3 sequence. Regions used as patient-specific CDR3 primers are highlighted in bold italics.