Characteristics of Clonotypic IgH VDJ Sequences
| Patient (status-150) . | Stage of Disease . | IgH . | Lt . | % PC-153 . | Vh Family . | Jh Family . | CDR3 Length (NT) . |
|---|---|---|---|---|---|---|---|
| 1. (Unt) | 3A | IgG | K | 23 | Vh2 (S12-12) | Jh4 (b) | 42 |
| 2. (Unt) | 3A | IgG | K | 31 | Vh5 (DP73) | Jh3 (a) | 30 |
| 3. (Tr) | Prog. | IgA | K | 33 | Vh3 (DP77) | Jh2 | 51 |
| 4. (Unt) | 3 | IgA | K | 30 | Vh4 (DP65) | nd | 36 |
| 5. (Unt) | 2A | IgG | L | 23 | VH3 (DP49) | Jh6 (b) | 48 |
| 6. (Unt) | 3 | IgG | K | 30 | Vh4 (DP71) | Jh4 (a) | 48 |
| 7. (Unt) | 3A | IgG | K | 25 | VH3 (DP77) | Jh2 | 48 |
| 8. (Off) | Relapse | IgA | L | 57 | Vh3 (DP46) | Jh3 (b) | 42 |
| 9. (Unt) | 3 | Nda | L | 80 | Vh3-21 (DP77) | Jh6 (c) | 51 |
| 10. (Unt) | Prog. | IgG | 90 | Vh2 (S12-12) | Jh4 (a) | 42 | |
| 11. (Ind) | 1A | IgA | K | 51 | Vh3-30 (DP49) | Jh4 (a) | 33 |
| 12. (Tr)-151 | Stable Resp. | IgG | L | 11 | Vh3-15 (DP389) | Jh4 (a) | 42 |
| 13. (Unt) | 3 | IgA | K | 70 | Vh3-8 (DP58) | Jh4 (a) | 39 |
| 14. (Off)-152 | Relapse | IgG | K | 67 | Vh3-30 (DP49) | Jh4 (b) | 39 |
| 15. (Unt) | 3 | IgG | K | 75 | Vh4-31 (DP78) | Jh4 (b) | 24 |
| 16. (Unt) | 3 | IgG | K | 22 | Vh1 (DP88) | Jh4 (b) | 51 |
| 17. (Unt) | 3A | IgG | K | 50 | Vh3-49 (DP57) | Jh4 (c) | 84 |
| 18. (Tr) | 3 | IgG | K | 41 | Vh5-51 (DP73) | Jh4 (b) | 27 |
| 19. (Unt) | 3 | IgG | K | 55 | Vh-3 (DP47) | Jh4 (b) | 45 |
| Patient (status-150) . | Stage of Disease . | IgH . | Lt . | % PC-153 . | Vh Family . | Jh Family . | CDR3 Length (NT) . |
|---|---|---|---|---|---|---|---|
| 1. (Unt) | 3A | IgG | K | 23 | Vh2 (S12-12) | Jh4 (b) | 42 |
| 2. (Unt) | 3A | IgG | K | 31 | Vh5 (DP73) | Jh3 (a) | 30 |
| 3. (Tr) | Prog. | IgA | K | 33 | Vh3 (DP77) | Jh2 | 51 |
| 4. (Unt) | 3 | IgA | K | 30 | Vh4 (DP65) | nd | 36 |
| 5. (Unt) | 2A | IgG | L | 23 | VH3 (DP49) | Jh6 (b) | 48 |
| 6. (Unt) | 3 | IgG | K | 30 | Vh4 (DP71) | Jh4 (a) | 48 |
| 7. (Unt) | 3A | IgG | K | 25 | VH3 (DP77) | Jh2 | 48 |
| 8. (Off) | Relapse | IgA | L | 57 | Vh3 (DP46) | Jh3 (b) | 42 |
| 9. (Unt) | 3 | Nda | L | 80 | Vh3-21 (DP77) | Jh6 (c) | 51 |
| 10. (Unt) | Prog. | IgG | 90 | Vh2 (S12-12) | Jh4 (a) | 42 | |
| 11. (Ind) | 1A | IgA | K | 51 | Vh3-30 (DP49) | Jh4 (a) | 33 |
| 12. (Tr)-151 | Stable Resp. | IgG | L | 11 | Vh3-15 (DP389) | Jh4 (a) | 42 |
| 13. (Unt) | 3 | IgA | K | 70 | Vh3-8 (DP58) | Jh4 (a) | 39 |
| 14. (Off)-152 | Relapse | IgG | K | 67 | Vh3-30 (DP49) | Jh4 (b) | 39 |
| 15. (Unt) | 3 | IgG | K | 75 | Vh4-31 (DP78) | Jh4 (b) | 24 |
| 16. (Unt) | 3 | IgG | K | 22 | Vh1 (DP88) | Jh4 (b) | 51 |
| 17. (Unt) | 3A | IgG | K | 50 | Vh3-49 (DP57) | Jh4 (c) | 84 |
| 18. (Tr) | 3 | IgG | K | 41 | Vh5-51 (DP73) | Jh4 (b) | 27 |
| 19. (Unt) | 3 | IgG | K | 55 | Vh-3 (DP47) | Jh4 (b) | 45 |
Abbreviations: ND, not detectable by routine clinical methods; Lt, light chain; K, kappa; L, lambda; Unt, untreated; Tr, treated; Off, Off therapy; Ind, indolent myeloma; Prog, progressive disease; Resp, responder.
These patients were all diagnosed with myeloma; patients 8 and 14 were in relapse at the time their BM was obtained. Patient 10 died 1 month after diagnosis. Their treatment status at the time the bone marrow sample was obtained is indicated.
This sample was taken immediately before autologous hematopoietic transplantation.
This patient was first diagnosed in 1988 and is thus a long-term survivor. In 1993/94, she had clonotypic DNA sequences detectable in her blood (4, Patient 3 in that study). The sequence of IgH VDJ transcripts in her plasma cells was determined at relapse for this study (in 1997) using single-sorted BM plasma cells. The CDR3 sequence obtained was identical to that determined previously.4 All of her relapse plasma cells express this sequence.
%PC = percent of plasma cells in the bone marrow sample used to derive the clonotypic sequence.