Ability of CD8+ Cells From CD28−/− Donors to Prevent Marrow Graft Rejection*
| Experiment . | CD8 Cells Added . | Percent Donor Marrow-Derived Cells . | |
|---|---|---|---|
| T Lymphocytes . | Granulocytes . | ||
| 1 | None | 1, 1, 0, 1 | 2, 3, 2, 3 |
| 8.0 × 105 CD28+/+ | 69, 62, 79, 69 | 98, 94, 94, 95 | |
| 8.0 × 105CD28−/− | 1, 0, 1, 0 | 1, 1, 1, 1 | |
| 3.2 × 106 CD28−/− | 1, 0, 0, 1 | 2, 1, 1, 1 | |
| 2 | None | 1, 0, 1, 0, 0 | 3, 4, 2, 1, 1 |
| 8.0 × 105 CD28+/+ | 65, 85, 73, 72 | 87, 95, 94, 96 | |
| 1.0 × 107CD28−/− | 88, 91, 90, 46 | 96, 95, 97, 89 | |
| Experiment . | CD8 Cells Added . | Percent Donor Marrow-Derived Cells . | |
|---|---|---|---|
| T Lymphocytes . | Granulocytes . | ||
| 1 | None | 1, 1, 0, 1 | 2, 3, 2, 3 |
| 8.0 × 105 CD28+/+ | 69, 62, 79, 69 | 98, 94, 94, 95 | |
| 8.0 × 105CD28−/− | 1, 0, 1, 0 | 1, 1, 1, 1 | |
| 3.2 × 106 CD28−/− | 1, 0, 0, 1 | 2, 1, 1, 1 | |
| 2 | None | 1, 0, 1, 0, 0 | 3, 4, 2, 1, 1 |
| 8.0 × 105 CD28+/+ | 65, 85, 73, 72 | 87, 95, 94, 96 | |
| 1.0 × 107CD28−/− | 88, 91, 90, 46 | 96, 95, 97, 89 | |
*Groups of four or five irradiated (550 cGy) bm1 recipients were transplanted with 5.0 × 106 T-cell–depleted B6-Ly5.1 marrow cells alone or together with the indicated number of CD8-enriched lymph nodes cells from CD28+/+ or CD28−/− B6 mice. The percent donor marrow-derived T cells and granulocytes in the peripheral blood on day 28 after transplantation was determined by two-color staining with CD3 and Ly5.1-specific antibodies. Data indicate results for individual recipients in two separate experiments. Data in experiment 2 were similar when results were analyzed on day 60 after transplantation (not shown).