Donor T Cells Without CD28 Were Unable to Expand and Delete Host Cells in BDF1 Recipients*
| Cell Type . | No Transplant . | CD28+/+ Transplant . | CD28−/− Transplant . | P Value-151 . |
|---|---|---|---|---|
| Donor | ||||
| CD4 | 0.1 ± 0.0-152 | 3.3 ± 1.7 | 0.2 ± 0.0 | .03 |
| CD8 | 0.0 ± 0.0 | 2.0 ± 0.9 | 0.1 ± 0.0 | .02 |
| B | 0.2 ± 0.1 | 1.2 ± 0.5 | 0.5 ± 0.2 | .05 |
| Host | ||||
| CD4 | 9.6 ± 1.7 | 2.8 ± 1.8 | 12.2 ± 0.4 | <.001 |
| CD8 | 4.9 ± 0.5 | 6.1 ± 2.1 | 5.7 ± 0.2 | .7 |
| B | 33.1 ± 3.4 | 0.5 ± 0.1 | 37.4 ± 2.6 | <.001 |
| Cell Type . | No Transplant . | CD28+/+ Transplant . | CD28−/− Transplant . | P Value-151 . |
|---|---|---|---|---|
| Donor | ||||
| CD4 | 0.1 ± 0.0-152 | 3.3 ± 1.7 | 0.2 ± 0.0 | .03 |
| CD8 | 0.0 ± 0.0 | 2.0 ± 0.9 | 0.1 ± 0.0 | .02 |
| B | 0.2 ± 0.1 | 1.2 ± 0.5 | 0.5 ± 0.2 | .05 |
| Host | ||||
| CD4 | 9.6 ± 1.7 | 2.8 ± 1.8 | 12.2 ± 0.4 | <.001 |
| CD8 | 4.9 ± 0.5 | 6.1 ± 2.1 | 5.7 ± 0.2 | .7 |
| B | 33.1 ± 3.4 | 0.5 ± 0.1 | 37.4 ± 2.6 | <.001 |
*Spleens were obtained from BDF1 mice with no transplant or from BDF1 recipients of CD28+/+ or CD28−/− donor cells 15 days after transplantation. Splenocytes were stained as shown in Fig 1. The values listed are absolute number of T or B cells (×10−6) (mean ± SE) per spleen, and represent the results of three mice per group.
P values represent the results of t-test comparisons of splenic lymphocyte populations in the recipients of CD28+/+ versus CD28−/− donor cells.
Donor-type cells in BDF1 mice with no transplant represent the background caused by nonspecific staining.