γ Globin Gene Expression in MEL Cell Pools Containing 10 or 50 Individual Clones Carrying the μLCR(−382)Aγ Construct
| No. of Clones per Pool . | Pool No. . | Copy No. . | % γ/α mRNA per Copy . |
|---|---|---|---|
| 10 clones | A | 20.6 | 1.5 |
| B | 3.5 | 4.0 | |
| C | 1.3 | 5.5 | |
| D | 2.3 | 9.9 | |
| E | 19.9 | 20.2 | |
| F | 9.0 | 21.1 | |
| Mean ± SD | 10.4 ± 8.4 | ||
| Range | 14.1x | ||
| 50 clones | A | 12.0 | 15.4 |
| B | 13.8 | 22.4 | |
| C | 5.3 | 27.7 | |
| D | 7.0 | 28.7 | |
| E | 6.2 | 30.9 | |
| F | 11.5 | 33.5 | |
| G | 6.9 | 33.6 | |
| Mean ± SD | 27.5 ± 6.6 | ||
| Range | 2.2x |
| No. of Clones per Pool . | Pool No. . | Copy No. . | % γ/α mRNA per Copy . |
|---|---|---|---|
| 10 clones | A | 20.6 | 1.5 |
| B | 3.5 | 4.0 | |
| C | 1.3 | 5.5 | |
| D | 2.3 | 9.9 | |
| E | 19.9 | 20.2 | |
| F | 9.0 | 21.1 | |
| Mean ± SD | 10.4 ± 8.4 | ||
| Range | 14.1x | ||
| 50 clones | A | 12.0 | 15.4 |
| B | 13.8 | 22.4 | |
| C | 5.3 | 27.7 | |
| D | 7.0 | 28.7 | |
| E | 6.2 | 30.9 | |
| F | 11.5 | 33.5 | |
| G | 6.9 | 33.6 | |
| Mean ± SD | 27.5 ± 6.6 | ||
| Range | 2.2x |