γ Globin Gene Expression in MEL Cell Pools Transfected With Four Aγ Promoter Truncation Mutants
| Construct . | Pool No. . | Copy No. . | % γ/α mRNA per Copy . |
|---|---|---|---|
| μLCR(−141)Aγ | A | 15.1 | 2.8 |
| B | 20.8 | 2.4 | |
| C | 9.2 | 4.8 | |
| D | 7.8 | 2.3 | |
| E | 8.2 | 2.5 | |
| F | 9.9 | 1.6 | |
| Mean ± SD | 2.7 ± 1.1 | ||
| Range | 3x | ||
| μLCR(−201)Aγ | A | 1.8 | 11.7 |
| B | 2.7 | 22.1 | |
| C | 2.6 | 73.0 | |
| D | 7.4 | 19.0 | |
| E | 6.2 | 29.6 | |
| F | 5.4 | 23.7 | |
| Mean ± SD | 29.9 ± 21.9 | ||
| Range | 6.2x | ||
| μLCR(−382)Aγ | A | 1.6 | 30.0 |
| B | 1 | 26.9 | |
| C | 2 | 29.6 | |
| D | 4.9 | 42.3 | |
| E | 7.8 | 29.6 | |
| F | 6.4 | 18.0 | |
| Mean ± SD | 29.4 ± 7.8 | ||
| Range | 2.4x | ||
| μLCR(−730)Aγ | A | 0.7 | 9.7 |
| B | 1.5 | 25.8 | |
| C | 2.5 | 96.4 | |
| D | 8.4 | 13.8 | |
| E | 1.4 | 18.6 | |
| F | 6.7 | 17.6 | |
| Mean ± SD | 30.3 ± 32.8 | ||
| Range | 9.9x |
| Construct . | Pool No. . | Copy No. . | % γ/α mRNA per Copy . |
|---|---|---|---|
| μLCR(−141)Aγ | A | 15.1 | 2.8 |
| B | 20.8 | 2.4 | |
| C | 9.2 | 4.8 | |
| D | 7.8 | 2.3 | |
| E | 8.2 | 2.5 | |
| F | 9.9 | 1.6 | |
| Mean ± SD | 2.7 ± 1.1 | ||
| Range | 3x | ||
| μLCR(−201)Aγ | A | 1.8 | 11.7 |
| B | 2.7 | 22.1 | |
| C | 2.6 | 73.0 | |
| D | 7.4 | 19.0 | |
| E | 6.2 | 29.6 | |
| F | 5.4 | 23.7 | |
| Mean ± SD | 29.9 ± 21.9 | ||
| Range | 6.2x | ||
| μLCR(−382)Aγ | A | 1.6 | 30.0 |
| B | 1 | 26.9 | |
| C | 2 | 29.6 | |
| D | 4.9 | 42.3 | |
| E | 7.8 | 29.6 | |
| F | 6.4 | 18.0 | |
| Mean ± SD | 29.4 ± 7.8 | ||
| Range | 2.4x | ||
| μLCR(−730)Aγ | A | 0.7 | 9.7 |
| B | 1.5 | 25.8 | |
| C | 2.5 | 96.4 | |
| D | 8.4 | 13.8 | |
| E | 1.4 | 18.6 | |
| F | 6.7 | 17.6 | |
| Mean ± SD | 30.3 ± 32.8 | ||
| Range | 9.9x |