PCR Primers Used to Clone LRP Fragment Constructs
| Construct . | Amino Acids . | Oligos . | |
|---|---|---|---|
| E4 | 783-830 | Fwd: | GCCGGATCCGTTGGCACCAACAAATGCCGG |
| Rev: | CGGCTCGAGCACGTAGGATGGGTTCGC | ||
| E4-C3 | 783-875 | Rev: | GCGCCTCGAGGGTGTGCTGATGGCAGAG |
| E4-C5 | 783-956 | Rev: | CGCCTCGAGGGTGGGATAGGCACACGAAGC |
| E4-C7 | 783-1036 | Rev: | CGGCTCGAGGGCCTGGTTGGTGCA |
| E4-C8 | 783-1082 | Rev: | CGGCTCGAGGGTCACTCCCTCACA |
| E4-C10 | 783-1165 | Rev: | GCGCCTCGAGCTGGTCGCAGAGCTCGCC |
| E4-E6 | 783-1246 | Rev: | GCGCCTCGAGGTCCAGGCTGCGGCAGCT |
| C5-C10 | 913-1165 | Fwd: | GCGGGATCCTCAGCCCGCACCTGC |
| Rev: | GCGCCTCGAGCTGGTCGCAGAGCTCGCC | ||
| C5 | 913-956 | Rev: | CGCCTCGAGGGTGGGATAGGCACACGAAGC |
| C5-C7 | 913-1036 | Rev: | CGGCTCGAGGGCCTGGTTGGTGCA |
| C6-C7 | 953-1036 | Fwd: | GCGGGATCCGCCTATCCCACCTGC |
| C9-E6 | 1079-1246 | Fwd: | GCGGGATCCGAGGGAGTGACCCAC |
| Rev: | GCGCCTCGAGGTCCAGGCTGCGGCAGCT | ||
| E5-1399 | 1164-1399 | Fwd: | GCGGGATCCGACCAGTGCTCTCTG |
| Rev: | TGCCTCGAGCATGGAGGCTGCCTCAATGCG | ||
| Construct . | Amino Acids . | Oligos . | |
|---|---|---|---|
| E4 | 783-830 | Fwd: | GCCGGATCCGTTGGCACCAACAAATGCCGG |
| Rev: | CGGCTCGAGCACGTAGGATGGGTTCGC | ||
| E4-C3 | 783-875 | Rev: | GCGCCTCGAGGGTGTGCTGATGGCAGAG |
| E4-C5 | 783-956 | Rev: | CGCCTCGAGGGTGGGATAGGCACACGAAGC |
| E4-C7 | 783-1036 | Rev: | CGGCTCGAGGGCCTGGTTGGTGCA |
| E4-C8 | 783-1082 | Rev: | CGGCTCGAGGGTCACTCCCTCACA |
| E4-C10 | 783-1165 | Rev: | GCGCCTCGAGCTGGTCGCAGAGCTCGCC |
| E4-E6 | 783-1246 | Rev: | GCGCCTCGAGGTCCAGGCTGCGGCAGCT |
| C5-C10 | 913-1165 | Fwd: | GCGGGATCCTCAGCCCGCACCTGC |
| Rev: | GCGCCTCGAGCTGGTCGCAGAGCTCGCC | ||
| C5 | 913-956 | Rev: | CGCCTCGAGGGTGGGATAGGCACACGAAGC |
| C5-C7 | 913-1036 | Rev: | CGGCTCGAGGGCCTGGTTGGTGCA |
| C6-C7 | 953-1036 | Fwd: | GCGGGATCCGCCTATCCCACCTGC |
| C9-E6 | 1079-1246 | Fwd: | GCGGGATCCGAGGGAGTGACCCAC |
| Rev: | GCGCCTCGAGGTCCAGGCTGCGGCAGCT | ||
| E5-1399 | 1164-1399 | Fwd: | GCGGGATCCGACCAGTGCTCTCTG |
| Rev: | TGCCTCGAGCATGGAGGCTGCCTCAATGCG | ||
Abbreviations: Fwd, forward; Rev, reverse.
LRP repeats have been numbered according to Herz et al,2where “E” represents an epidermal growth factor-type repeat and “C” represents a complement-type repeat. Restriction sites (BamHI or Xho I) are underlined. Overhangs of several bases were included at the 5′ ends of the primers to facilitate restriction enzyme cleavage.