Oligonucleotide Probes Used in EMSAs
| Name . | Sequence 5′3′ . |
|---|---|
| Irrelevant probe | |
| Irr. | GGAAGCAGACCACGTGGTCTGCTTCC |
| Probes for Sp1 region-150 | |
| MS6 | TCCCCTCCCCTCCCCTCC |
| MS7 | CCGTTGCGTCCCACCCACCGCGT |
| mMS7 | CCGTTGCGTCCCAttCACCGCGT |
| Probes for megakaryocytic cell-specific activity region | |
| −126−115−108 −90 | |
| ‖‖‖‖ | |
| MSPw | CGGAGGGGAGGAAGCGCGAGTCCGCG |
| Pw | CTAGAGAAGCCGGAGGGGAGGAAGCGC |
| mPw1 | CTAGAGAAGCCGGcGttGAGGAAGCGC |
| mPw2 | CTAGAGAAGaCtGAGGGGAGGAAGCGC |
| Pws | AAGCCGGAGGGGAGG |
| MS4 | AGGGGAGGAAGCGCGAGTCC |
| mMS4 | AGGGGcGtcAGCGCGAGTCC |
| Name . | Sequence 5′3′ . |
|---|---|
| Irrelevant probe | |
| Irr. | GGAAGCAGACCACGTGGTCTGCTTCC |
| Probes for Sp1 region-150 | |
| MS6 | TCCCCTCCCCTCCCCTCC |
| MS7 | CCGTTGCGTCCCACCCACCGCGT |
| mMS7 | CCGTTGCGTCCCAttCACCGCGT |
| Probes for megakaryocytic cell-specific activity region | |
| −126−115−108 −90 | |
| ‖‖‖‖ | |
| MSPw | CGGAGGGGAGGAAGCGCGAGTCCGCG |
| Pw | CTAGAGAAGCCGGAGGGGAGGAAGCGC |
| mPw1 | CTAGAGAAGCCGGcGttGAGGAAGCGC |
| mPw2 | CTAGAGAAGaCtGAGGGGAGGAAGCGC |
| Pws | AAGCCGGAGGGGAGG |
| MS4 | AGGGGAGGAAGCGCGAGTCC |
| mMS4 | AGGGGcGtcAGCGCGAGTCC |
The indicated sequences represent the double-stranded probe resulting after annealing of sense and antisense oligonucleotides, followed by a fill-in reaction. Probes are aligned when appropriate. Mutant sequences are indicated by bold lowercase letters. The position of the sequences relative to the transcription start site is shown for the last group of probes.
The Sp1 consensus binding site in MS7 is underlined.