Table 2. Effect of Cell Cycle... | American Society of Hematology

Table 2.

Effect of Cell Cycle Progression Under IL-3-SCF-FL Stimulation on the Repopulating Activity of CD34⁺ Cells Initially Isolated in G₀

Experiment	Time in Culture	Cell Cycle Status	Cell Dose/Mouse (×10⁶)	% Chimerism (%CD45⁺)^*
1	0 h	G₀	1.0	52.2; 20.8; 68.7
	36 h	G₀	1.0	35.2; 38.1
		G₁	1.0	1.6; 0.2
2	0 h	G₀	0.7	12.0; 18.0
	36 h	G₀	0.7	5.2; 9.15
		G₁	0.7	<0.1; <0.1
		Total	0.7	<0.1; 0.4
3	0 h	G₀	0.7	12.9
	36 h	Total	0.7	1.5; 3.4

Experiment	Time in Culture	Cell Cycle Status	Cell Dose/Mouse (×10⁶)	% Chimerism (%CD45⁺)^*
1	0 h	G₀	1.0	52.2; 20.8; 68.7
	36 h	G₀	1.0	35.2; 38.1
		G₁	1.0	1.6; 0.2
2	0 h	G₀	0.7	12.0; 18.0
	36 h	G₀	0.7	5.2; 9.15
		G₁	0.7	<0.1; <0.1
		Total	0.7	<0.1; 0.4
3	0 h	G₀	0.7	12.9
	36 h	Total	0.7	1.5; 3.4

NOD/SCID mice were transplanted with indicated cell doses of freshly isolated (0 h) G₀CD34⁺ cells or with CD34⁺ cells initially isolated in G₀ and either remaining in G₀ or progressing into G₁ after 36 hours of ex vivo culture under IL-3-SCF-FL stimulation, as shown in Fig5. In experiments 2 and 3, the total represents CD34⁺ cells isolated in G₀, cultured for 36 hours, and directly assayed for NOD/SCID repopulating capacity without restaining with Hst/PY and cell cycle fractionation.

Percentage of CD45⁺ cells are indicated for each recipient mouse 6 weeks posttransplantation.

View Large

This Feature Is Available To Subscribers Only