PCR Analysis of Myeloma Patient Stroma Grown in Various Culture Conditions and Concurrent Serological Testing for HHV-8 Antibody
| Patient ID . | Disease Status . | Cells Cultured* . | PCR Results† . | Serum HHV-8 IgG by IFA . | ||
|---|---|---|---|---|---|---|
| D10 . | LTCM . | Dexter . | ||||
| N1 | PR | Fresh | Neg | NT | NT | Neg |
| N2 | PR | Fresh | Neg | NT | NT | NT |
| N3 | PR | Fresh | Neg | NT | NT | Neg |
| N4 | PR | Fresh | Neg | NT | NT | Neg |
| N5 | PR | Frozen | Neg | Neg | NT | Neg |
| PD-T | Fresh | Neg | Neg | NT | ||
| N6 | PR | Frozen | Pos | NT | NT | NT |
| CR-T | Fresh (d10) | Pos | Neg | NT | Neg | |
| Fresh (d21) | Pos | Neg | NT | |||
| N7 | PR | Fresh | Neg | Neg | NT | Neg |
| N8 | PR | Frozen | Neg | Neg | NT | NT |
| N9 | PR | Fresh | Pos | Neg | NT | NT |
| N10 | PD | Fresh | NT | Neg | NT | NT |
| N11 | PD | Fresh | Pos | Neg | Pos | Neg |
| N12 | PR | Fresh | Neg | Neg | Neg | Neg |
| N13 | PR-T | Fresh | Neg | Pos | Neg | Neg |
| N14 | PR | Fresh | Neg | Pos | Neg | Neg |
| N15 | PR | Fresh | Neg | Neg | Neg | Neg |
| Patient ID . | Disease Status . | Cells Cultured* . | PCR Results† . | Serum HHV-8 IgG by IFA . | ||
|---|---|---|---|---|---|---|
| D10 . | LTCM . | Dexter . | ||||
| N1 | PR | Fresh | Neg | NT | NT | Neg |
| N2 | PR | Fresh | Neg | NT | NT | NT |
| N3 | PR | Fresh | Neg | NT | NT | Neg |
| N4 | PR | Fresh | Neg | NT | NT | Neg |
| N5 | PR | Frozen | Neg | Neg | NT | Neg |
| PD-T | Fresh | Neg | Neg | NT | ||
| N6 | PR | Frozen | Pos | NT | NT | NT |
| CR-T | Fresh (d10) | Pos | Neg | NT | Neg | |
| Fresh (d21) | Pos | Neg | NT | |||
| N7 | PR | Fresh | Neg | Neg | NT | Neg |
| N8 | PR | Frozen | Neg | Neg | NT | NT |
| N9 | PR | Fresh | Pos | Neg | NT | NT |
| N10 | PD | Fresh | NT | Neg | NT | NT |
| N11 | PD | Fresh | Pos | Neg | Pos | Neg |
| N12 | PR | Fresh | Neg | Neg | Neg | Neg |
| N13 | PR-T | Fresh | Neg | Pos | Neg | Neg |
| N14 | PR | Fresh | Neg | Pos | Neg | Neg |
| N15 | PR | Fresh | Neg | Neg | Neg | Neg |
Abbreviations: PR, partial remission; PD, progressive disease; CR-T, complete remission after transplantation; PR-T, partial remission after transplantation; PD-T, progressive disease after transplantation; NT, not tested.
Fresh or frozen bone marrow mononuclear cells were cultured under different parallel conditions (D10, LTCM, and Dexter, see Materials and Methods for details) and adherent cells analyzed at 3 weeks unless otherwise stated. d10, analyzed on day 10; d21, analyzed on day 21.
PCR was performed using primers from the orf 26 (KS330) region.