Table 1.

PCR Analysis of Myeloma Patient Stroma Grown in Dexter Cultures and Concurrent Serological Testing for HHV-8 Antibody

Patient ID Disease Status Cells Cultured-150PCR Results-151 (no. positive/ no. tested) Serum HHV-8 IgG by IFA
T1  PR  Fresh  1/3  Neg  
T2 PR  Fresh  1/3  Neg  
T3  PR  Fresh  2/3 Neg  
T5  PR  Fresh  2/2  NT  
T6  PR  Fresh 1/3  Neg  
T7  PR  Fresh  2/3  Neg  
T8  PR Fresh  2/3  Neg  
T9  PR  Fresh  3/3  Neg 
T10  PR  Fresh  2/3  NT  
T11  PR  Fresh 1/3  Neg  
T12  PR  Fresh  0/3  Neg  
T13  PR Fresh  1/3  NT  
T14  PR  Fresh  1/3  NT 
T15  PR  Fresh  2/3  NT  
T16  PR  Fresh 0/2  NT 
Patient ID Disease Status Cells Cultured-150PCR Results-151 (no. positive/ no. tested) Serum HHV-8 IgG by IFA
T1  PR  Fresh  1/3  Neg  
T2 PR  Fresh  1/3  Neg  
T3  PR  Fresh  2/3 Neg  
T5  PR  Fresh  2/2  NT  
T6  PR  Fresh 1/3  Neg  
T7  PR  Fresh  2/3  Neg  
T8  PR Fresh  2/3  Neg  
T9  PR  Fresh  3/3  Neg 
T10  PR  Fresh  2/3  NT  
T11  PR  Fresh 1/3  Neg  
T12  PR  Fresh  0/3  Neg  
T13  PR Fresh  1/3  NT  
T14  PR  Fresh  1/3  NT 
T15  PR  Fresh  2/3  NT  
T16  PR  Fresh 0/2  NT 

Abbreviations: PR, partial remission; NT, not tested.

F0-150

Fresh or frozen cells were cultured for 3 weeks under standard Dexter culture conditions and adherent cells were analyzed.

F0-151

PCR was performed using primers from the orf 26 (KS330) region.

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