Table 1. HLA-DRB1*0901-Restricted... | American Society of Hematology

Table 1.

HLA-DRB1*0901-Restricted Proliferative Responses of bcr-abl b3a2-Specific CD4⁺ T-Cell Clones

Exp. 1^-150 Clone	APC	MoAb Added	Peptide Stimulation	Proliferative Response (cpm) (mean ± SD)
MY-1	auto PBMC	None	None	259 ± 37
			b3a2	26,771 ± 2,628
			b2a2	324 ± 28
			b3b4	332 ± 31
			1A-a2	300 ± 29
		anti–HLA-DR	b3a2	449 ± 69
		anti–HLA-DQ	b3a2	26,678 ± 3,326
		anti–HLA-DP	b3a2	26,139 ± 4,559
TO-1	auto PBMC	None	None	219 ± 24
			b3a2	31,309 ± 3,237
			b2a2	279 ± 102
			b3b4	145 ± 6
			1A-a2	157 ± 11
		anti–HLA-DR	b3a2	308 ± 200
		anti–HLA-DQ	b3a2	25,664 ± 523
		anti–HLA-DP	b3a2	29,731 ± 2,632

Exp. 1^-150 Clone	APC	MoAb Added	Peptide Stimulation	Proliferative Response (cpm) (mean ± SD)
MY-1	auto PBMC	None	None	259 ± 37
			b3a2	26,771 ± 2,628
			b2a2	324 ± 28
			b3b4	332 ± 31
			1A-a2	300 ± 29
		anti–HLA-DR	b3a2	449 ± 69
		anti–HLA-DQ	b3a2	26,678 ± 3,326
		anti–HLA-DP	b3a2	26,139 ± 4,559
TO-1	auto PBMC	None	None	219 ± 24
			b3a2	31,309 ± 3,237
			b2a2	279 ± 102
			b3b4	145 ± 6
			1A-a2	157 ± 11
		anti–HLA-DR	b3a2	308 ± 200
		anti–HLA-DQ	b3a2	25,664 ± 523
		anti–HLA-DP	b3a2	29,731 ± 2,632

Exp. 2^-151 Clone	APC	Peptide Stimulation	Proliferative Response (cpm) (mean ± SD)
MY-1	None	None	210 ± 52
		b3a2	870 ± 212
	L-Neo	None	214 ± 20
		b3a2	702 ± 145
	L-DR9	None	248 ± 48
		b3a2	24,208 ± 507
	L-DR4	None	254 ± 79
		b3a2	799 ± 97
TO-1	None	None	268 ± 30
		b3a2	936 ± 62
	L-Neo	None	262 ± 34
		b3a2	893 ± 22
	L-DR9	None	308 ± 50
		b3a2	29,760 ± 2,554
	L-DR4	None	358 ± 27
		b3a2	723 ± 54
None	L-DR9	None	227 ± 25
		b3a2	367 ± 188

Exp. 2^-151 Clone	APC	Peptide Stimulation	Proliferative Response (cpm) (mean ± SD)
MY-1	None	None	210 ± 52
		b3a2	870 ± 212
	L-Neo	None	214 ± 20
		b3a2	702 ± 145
	L-DR9	None	248 ± 48
		b3a2	24,208 ± 507
	L-DR4	None	254 ± 79
		b3a2	799 ± 97
TO-1	None	None	268 ± 30
		b3a2	936 ± 62
	L-Neo	None	262 ± 34
		b3a2	893 ± 22
	L-DR9	None	308 ± 50
		b3a2	29,760 ± 2,554
	L-DR4	None	358 ± 27
		b3a2	723 ± 54
None	L-DR9	None	227 ± 25
		b3a2	367 ± 188

Abbreviation: Exp., experiment.

F0-150

Incorporation of ³H-TdR into cloned T cells was determined in the presence of autologous APCs with and without various types of synthetic peptide and with and without anti-HLA MoAb.

F0-151

Incorporation of ³H-TdR into cloned T cells was determined in the presence and absence of various types of APC with and without b3a2 peptide.

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