Cell Lines Established From Mcl-1 Transgenic Mice
| Cell Line* (tissue of origin) . | Months in Culture (months survival of nontransgenic control) . |
|---|---|
| Nonadherent mast cell lines | |
| UU3SP (spleen)1-153 | 5† (<1) |
| O4SP (spleen) | 6† (1.51-155) |
| I2SP (spleen) | 2† (1.51-155) |
| 4Q6SP (spleen) | 22† (3) |
| 4Q6BM (bone marrow)1-153 | 22† (4) |
| 3Y10BM (bone marrow) | 7† (<1) |
| 4W2BM (bone marrow) | 6† (3) |
| 5C2BM (bone marrow) | 13‡ (5) |
| 5B4BM (bone marrow) | 13‡ (6) |
| Adherent monocytic cell lines | |
| 4Q6BM-Adh (bone marrow)1-153 | 8‡ (4) |
| UU3SP-Adh (spleen)1-153 | 18‡ (<1) |
| E2SP-Adh (spleen) | 24‡ (ND) |
| Cell Line* (tissue of origin) . | Months in Culture (months survival of nontransgenic control) . |
|---|---|
| Nonadherent mast cell lines | |
| UU3SP (spleen)1-153 | 5† (<1) |
| O4SP (spleen) | 6† (1.51-155) |
| I2SP (spleen) | 2† (1.51-155) |
| 4Q6SP (spleen) | 22† (3) |
| 4Q6BM (bone marrow)1-153 | 22† (4) |
| 3Y10BM (bone marrow) | 7† (<1) |
| 4W2BM (bone marrow) | 6† (3) |
| 5C2BM (bone marrow) | 13‡ (5) |
| 5B4BM (bone marrow) | 13‡ (6) |
| Adherent monocytic cell lines | |
| 4Q6BM-Adh (bone marrow)1-153 | 8‡ (4) |
| UU3SP-Adh (spleen)1-153 | 18‡ (<1) |
| E2SP-Adh (spleen) | 24‡ (ND) |
Abbreviation: ND, not determined.
A cell inoculum containing a total of 5 × 106 cells (in 10 mL) was maintained in standard medium (10% FBS) for 4 days, and then continuously cultured in the presence of IL-3 as described in Materials and Methods, except for cell lines 5C2BM and 5B6BM which were placed directly into IL-3–containing medium.
Transgenic lines terminated voluntarily.
Transgenic lines still in culture.
The pair of clones UU3SP and UU3SP-Adh, as well as the pair 4Q6BM and 4Q6BM-Adh, were derived by separately culturing adherent versus nonadherent cells from a single mouse.
Cells were cultured simultaneously from two separate transgenic mice, with a single nontransgenic mouse serving as the control for both.