IL-4 Production in Primary Cultures
| . | Exp. 1 . | Exp. 2 . | Exp. 3 . | Exp. 4 . | Exp. 5 . |
|---|---|---|---|---|---|
| Nil | 0.68 | 1.16 | 0.73 | <0.25 | 5.14 |
| mIgG2a + mIgG2a | 0.47 | 0.69 | 1.03 | <0.25 | 4.95 |
| mIgG2a + anti-OX40 | 0.95 | 1.93 | 1.30 | 3.14 | 4.52 |
| Anti–IL-4R + mIgG2a | 23.53 | 15.18 | 14.14 | 1.20 | 100.20 |
| Anti–IL-4R + anti-OX40 | 40.32 | 28.86 | 23.16 | 21.08 | 158.03 |
| . | Exp. 1 . | Exp. 2 . | Exp. 3 . | Exp. 4 . | Exp. 5 . |
|---|---|---|---|---|---|
| Nil | 0.68 | 1.16 | 0.73 | <0.25 | 5.14 |
| mIgG2a + mIgG2a | 0.47 | 0.69 | 1.03 | <0.25 | 4.95 |
| mIgG2a + anti-OX40 | 0.95 | 1.93 | 1.30 | 3.14 | 4.52 |
| Anti–IL-4R + mIgG2a | 23.53 | 15.18 | 14.14 | 1.20 | 100.20 |
| Anti–IL-4R + anti-OX40 | 40.32 | 28.86 | 23.16 | 21.08 | 158.03 |
IL-4 was measured after 72 hours of anti-CD3/B7.1 stimulation by means of Quantikine HS IL-4 ELISA. All antibodies were used at 5 μg/mL. Values are given as picograms per milliliter.